Greener Journal of Biochemisty and Biotechnology

Greener Journal of Biochemistry and Biotechnology Vol. 5 (1), pp. 001-008, 2018.

  2384-6321© 2018 

Research Paper

Manuscript Number: 062318068

 

 

Breaking of Seed Dormancy in Morindalucida Benth

 

 

¹*AJONGBOLO Febisola Bukola, ²OYETUNJI Olusola J, ³JAMALEDDINE Zainab Olubunmi,

⁴ADEDEJI Abdulkabir Adeyemi

 

 

¹National Centre for Genetic Resources and Biotechnology, Moor Plantation Apata, Ibadan.

²Department of Botany, University of Ibadan.

³National Centre for Genetic Resources and Biotechnology, Moor Plantation Apata, Ibadan.

⁴National Centre for Genetic Resources and Biotechnology, Moor Plantation Apata, Ibadan.

Abstract

The study was conducted to establish the best method for breaking seed dormancy in M. lucida via in vitro propagation, using scarification methods and hormone (GA₃) treatments to enhance uniform germination of plantlets for subsequent mass propagation. Seeds soaked in warm water for ten minutes then nicked at the micropylar region, gave optimum germination percentage when inoculated on growth medium. M. lucida seeds treated with 500mg/L GA3 gave the best plant height while number of shoots were observed to be highest on seeds scarified by nicking and treated with GA 3 at 100 and 300mg^-L .Observations on the morphological characteristics revealed that uniform germination was analogous in plantlets obtained from seeds scarified with either warm water or nicking while significantly higher germination was recorded for warm water coupled with nicking.

Keywords: Morinda lucida, GA₃- Gibberellic acid, In vitro propagation, MS-Murashige and Skoog medium, H₂SO₄- Sulphuric Acid, Hormone, Scarification

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DOI Link: http://doi.org/10.15580/GJBB.2018.1.062318068

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