Greener Journal of Agricultural Sciences

Greener Journal of  Agricultural Sciences Vol. 4 (2), pp. 039-045, March 2014.

 ISSN: 2276-7770 

Research Paper

Manuscript Number: 021014101

DOI: http://dx.doi.org/10.15580/GJAS.2014.2.021014101

 

Determination of Phaseolin Types  in Common Bean (Phaseolus vulgaris) Varieties from Turkey

                                                                                                     

Seher Yıldız Madakbaş¹*, Halima Awale² and

James Kelly²

 

¹Ahi Evran University, Agricultural Faculty, Department of Horticulture, Kırşehir-Turkey.

²Michigan State University, Department of Crop, Soil and Microbial Sciences, Michigan- USA.

 

*Corresponding Author’s Email: seheryldzmadakbas @ gmail.com

Abstract:

The purpose of our study was to determine the phaseolin types of common bean varieties widely consumed in Turkey through protein extraction. Twenty- four bean genotypes, consisting of 22 varieties from Kırsehir province of Turkey and two control varieties (Andean and Mesoamerican) were assessed. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) was performed with protein samples extracted from bean genotypes. Gels were stained with Coomassie blue dye R-250 and kept in fixing solution. Our analyses revealed that out of 22 bean samples, 16 contained Andean phaseolin type and 6 contained Mesoamerican phaseolin type. T-test statistical analysis was performed to determine the relationship between seed phaseolin types and seed volumes (length, height and width). The results showed that there was a significant difference between phaseolin and seed volume.
 
Keywords: Phaseolus vulgaris, phaseolin, SDS-PAGE, genotype, Andean, Mesoamerican, seed volume.

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