Greener Journal of Agricultural Sciences

Greener Journal of  Agricultural Sciences Vol. 4 (4), pp. 110-116, May 2014.

 ISSN: 2276-7770 

Research Paper

Manuscript Number: 012314055

DOI: http://dx.doi.org/10.15580/GJAS.2014.4.012314055

 

Introduction of the neMDH gene in Urochloa brizantha for Aluminium Tolerance in Agronomic Assays

 

Flavio Rocha¹, Keila Maria Roncato Duarte*²,

Luiz Humberto Gomes¹, Waldssimiller Teixeira Mattos³,

Simone Possedente Lira¹, Paulo Bardauil Alcantara³

 

¹Laboratório de Química Orgânica de Produtos Naturais, Departamento de Ciências Exatas, Escola Superior de Agricultura “Luiz de Queiroz”, USP, Piracicaba, 13418-900, São Paulo, Brazil.

²CPD Genetic and Animal Reproduction, Institute of Animal Science and Pastures, APTA/SAA Nova Odessa, 13460-000, São Paulo, Brazil.

³CPD Pastures and Nutrition , Institute of Animal Science and Pastures,

APTA/SAA Nova Odessa, 13460-000, São Paulo, Brazil.

 

*Corresponding Author’s Email: keiladuarte @ globo. com

Abstract:

Agricultural production in acid soils constituting about half of the total arable land on Earth, is limited by aluminium toxicity, which is manifested by root growth reduction, decreasing nutrients and water absorption by the plant. This work evaluated aluminium resistance in genotypes of Brachiaria brizantha cv. MG4, transformed with the neMDH gene, by using a nutrient solution for agronomic evaluation and gene integration confirmation by PCR. Three from the five confirmed transformed genotypes of B. brizantha cv. MG4 with the neMDH gene presented tolerance to aluminium solutions (111 µmol L ^-1 and 444 µmol L ^-1). Two from those three genotypes  also showed higher dry weight production in comparison to the wild-type genotype.

Keywords: Brachiaria, Tolerance, Aluminum, Soil, Transgenic, neMDH.

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Reference:

Anoop VM,  Basu U, Mccammon, MT, Mcalister-Henn L, Taylor G J (2003) . Modulation of citrate metabolism alters aluminum tolerance in yeast and transgenic canola overexpressing a mitochondrial citrate synthase. Plant Physiol 13: 2205‑2217. doi: ​10.​1104/​pp.​103.​023903

De La Fuente JM, Ramírez-Rodríguez V, Cabrera-Ponce J L, Herrera-Estrella L (1997). Aluminun tolerance in transgenic plants by alteration of citrate synthesis. Science, 276: 1566-1568. . DOI: 10.1126/science.276.5318.1566

Delhaize E, Ryan PR, Hebb DM, Yamamoto Y, Sasaki T, Matsumoto H (2004). Engineering high-level aluminum tolerance in barley with the ALMT1 gene. Proceedings of National Academy of Science of USA, 101: 15249–15254. doi:10.1073/pnas.0406258101

Doyle JJ, Doyle J L (1987). A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19: 11–15.

Furlani AMC, Furlani  P R (1988). Composição e pH de soluções nutritivas para estudos fisiológicos e seleção de plantas em condições nutricionais adversas. Boletim técnico, Instituto Agronômico, Campinas, 121, 34p.

Jackson SA, Zhang P, Chen WP, Phillips RL, Friebe B, Muthukrishnan S, Gill BS. (2001). High-resolution structural analysis of biolistic transgene integration into the genome of wheat. Theoretical and Applied  Genetics 103: 56-62. DOI:10.1007/s001220100608

Kochian LV, Hoekenga OA, Piñeros MA (2004). How do crop plants tolerate acid soils? Mechanisms of aluminum tolerance and phosphorus efficiency. Annu. Rev. Plant Biol  55:  459‑93. doi: 10.1146/annurev.arplant.55.031903.141655

Kochian, LV (1995). Cellular mechanisms of Al toxicity and resistance in plants. Ann Rev Plant Physiol Plant Mol Biol 46: 237-260. DOI: 10.1146/annurev.pp.46.060195.001321

Kohli A, Gahakwa D, Vain P,  Laurie DA, Christou P (1999). Transgene expression in rice engineered though particle bombardment: molecular factors controlling stable expression and transgene silencing. Planta  208: 88-97. DOI: 10.1007/s004250050538

Lessard PA, Kulaveerasingam H, York GM,  Strong A, Sinskey AJ (2002) Manipulation gene expression for the metabolic engineering of plants. Metabolic Engineering  4: 67-79. Doi:10.1006/mben.2001.0210

Macedo, MCM (2006). Aspectos Edáficos Relacionados com a produção de Brachiaria brizantha cultivar Marandu, In: BARBOSA R A Morte de pastos de braquiárias. Campo Grande, Embrapa Gado de Corte, 35-65.

Mazzocato ACC, Rocha PSG, Sereno MJCM, Bohnen H, Grongo V, Neto J F B ( 2002). Tolerância ao alumínio em plântulas de milho. Ciência Rural, Santa Maria 32: 19-24.

McNeilly T (1994) Metal toxicity, In: YEO AR, FLOWERS TJ Soil Mineral Stresses: Approaches to crop improvement: Monographs on Theoretical and Applied Genetics, Springer‑Verlag,  Berlin, 21: 145‑74.

Miller SS, Driscoll BT, Gregerson RG, Gantt JS, Vance CP (1998). Alfalfa malate dehydrogenase (MDH): molecular cloning and characterization of five different forms reveals a unique nodule-enhanced MDH. Plant Journal 15(2): 173-184.  DOI:10.1046/j.1365-313X.1998.00192

Minárik, P, Tomásková N, Kollárová M, Antalík M (2002). Malate dehydrogenases – structure and function. Gen Physiol Biophys 21: 257-265. doi:10.1186/1471-2229-13-23

Panda SK,  Baluska F, Matsumoto H (2009). Aluminum stress signaling in plants. Plant Signaling & Behavior 4( 7): 592-597.

Petry, D. T. 2009. Transformação de soja [Glycine max (L.) Merril] com plasmídeos e cassetes gênicos contendo o gene chit1 de Metarhizium anisopliae visando a obtenção de plantas resistentes a doenças fúngicas. 65p. Tese (Mestrado) – UFRGS.

Portaluppi R,  Brammer SP, Magalhaes JV, Costa CT, Caierao  E, Nascimento Junior A, Silva Junior JP (2010). Tolerância de genótipos de cereais de inverno ao alumínio em cultivo hidropônico e em campo. Pesq agropec bras 45: 178-185.

Quecini V, Moreti RMR, Duarte KMR, Alcantara VBG, Lucena MAC, Colombo CA, Siqueira WJ, Alcantara PB (2008). Over-expression of tricarboxilic acid metabolism genes in forage crops Neonotonia wightii and Brachiaria brizantha leads to ectopic root development, In: XXI International Grassland Congress, Hohhot. Multifunctional Grassland in a Changing World. 2: 389-389.

Ryan PR, Delhaize E, Randall P J (1995). Characterization of Al‑stimulated efflux of malate from apices of Al‑tolerant wheat roots. Planta 196: 103‑110. DOI:10.1007/BF00193223

Silva JAG, Carvalho FIF, Coimbra JLM, Vieira EA, Benin G, Oliveira AC, Finatto T, Bertain I, Silva GO, Correa MR (2007). Tolerância ao alumínio em cultivares de aveia branca sob cultivo hidropônico. Bragantia, Campinas 66(4): 587-593.

Tesfaye M, Temple SJ, Allan DL, Vance CP, Samac DA (2001) Overexpression of malate dehydrogenase in transgenic alfalfa enhances organic acid synthesis and confers tolerance to aluminum. Plant Physiol 127: 1836‑44. DOI: 10.3410/f.1003449.36154

Wenzl P, Mancilla LI, Mayer J E,  Albert R, Rao IM ( 2003). Simulating infertile acid soils with nutrient solutions: the effects on Brachiaria species. Soil Sci Soc Am Jour 67: 1457-1469.