Ali Et Al


Ali et al

Journal of  Agricultural Sciences

Vol. 3 (11), pp.
November 2013.

 ISSN: 2276-7770 



Manuscript Number: EB11091313


Effect of Different Levels of Organic Acids
Supplementation on Feed Intake, Milk Yield and Milk
Composition of Dairy Cows during Thermal Stress


Amjed Ali1, Sarzamin Khan*1,
Muhammad Mobashar1,

Muhammad Inam1, Iftikhar Ahmed1,
Nazir Ahmed Khan1 and Mubarak Ali2, 3,
Hamayun Khan4


of Poultry Science, the University of Agriculture,

Nutrition Group, Wageningen University, P.O. Box 338,
6700, AH Wageningen,

the Netherlands.

UR Livestock Research, P.O. Box 65, 8200 AB Lelystad,
the Netherlands.

of Animal Health, the University of Agriculture,
Peshawar, Pakistan.


*Corresponding Author’s Email: dr.zaminaup @


In many developing countries in the tropics, thermal stress
results in lower feed intake, changes in energy metabolism,
alterations in endocrine profiles of dairy cows which lead
to animal health problems and production losses.
Supplementation of organic acids can reduce the toxic
metabolites and endocrine changes in the cow’s body during
thermal stress. The present study was conducted at
University Dairy Farm, The University of Agriculture,
Peshawar to determine the effect of supplementing different
levels of organic acids solution in drinking water on the
milk yield, milk composition, milk somatic cell count (SCC),
blood haemoglobin (Hb) and packed cell volume (PCV) of dairy
cows during thermal stress. The solution of organic acids
was prepared by dissolving 80g citric acid, 90g phosphoric
acid, 50g lactic acid and 10g copper sulphate in one litre
of distilled water. Twelve Holstein Friesian dairy cows
(mid-lactation, 3 to 4 years old) having average body weight
of 400kg were blocked into four groups on the basis of
parity and milk yield. The cows were offered water without
(group A) or with organic acids solution at the rate of 0.5,
1 or 1.5ml per litre of drinking water. The experiment was
continued for 30 days along with two weeks adaptation period
during the summer months of July and August, 2012. The
addition of organic acids to the drinking water increased
(P<0.05) feed intake and milk yield. The addition of organic
acids significantly increased (P<0.05) the percentage of
fat, protein, lactose, solids not fat and total solids in
milk, whereas, the SCC decreased. The blood Hb and PCV
increased (P<0.05) with the use of organic acids in the
drinking water. The results of present research demonstrate
that the using organic acids solution in the drinking water
of dairy cows can maintain animal health and production
during thermal stress and thereby minimizing the production
losses and improve the profitability of the dairy farms.
Keywords: Thermal stress, organic acids, feed intake,
milk yield, milk composition, dairy cow.
Abbreviation: DMI, dry matter intake; Hb, haemoglobin;
PCV, packed cell volume; SNF, solid not fat; TS, total
solids; THI, Temperature Humidity Index.

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