Bakshi And Bhagwat


Bakshi and Bhagwat

Greener Journal of Agricultural Sciences Vol. 6 (11), pp. 349-355, December 2016.

 ISSN: 2276-7770 

Research Articles

Manuscript Number: 120816211



High Moleular Weight Glutenin Subunit Variation among Indian
Wheat Varieties


Bakshi Suman* and Bhagwat SG


Nuclear Agriculture
and Biotechnology Division, Bhabha Atomic Research Centre, Trombay,
Mumbai-400085, India.


wheat for end use quality is an important goal for wheat breeders. The
composition of high molecular weight glutenin subunits (HMW-GS) contributes to
a great extent in determining wheat quality for processing. The HMW-GS are
known to be encoded by six genes located at the Glu-1 loci on the long arms of chromosomes 1A, 1B and 1D. The
endosperm storage proteins of 62 Indian bread wheat cultivars were fractionated
by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) to
determine their high molecular weight (HMW) glutenin subunit composition. In
total, three alleles (Ax2*, Ax1 or Ax null) at Glu-A1, seven allele/allelic pairs (Bx7, Bx7+By8, Bx7+By9, Bx20,
Bx13+By16, Bx13+By19 and Bx17+By18) at Glu-B1
and two allelic pairs (Dx2+Dy12 and Dx5+Dy10) at Glu-D1 were identified in the
analysed varieties. Allele or allelic pair Ax2* (59.7%) at Glu-A1, Bx17+By18 (27.4%) at Glu-B1
and Dx2+Dy12 (69.4%) at Glu-D1 were
present most frequently in wheat
varieties taken
for study.

score of varieties were ranged from 4 to 10, with an average of 7.55 indicating
vast genetic diversity among the varieties. The presence of subunits
in different varieties with low, medium and high gluten
strength indicated that breeding wheat for bread, chapatti and biscuits is
possible as per end use requirement.


Key words: Bread wheat, SDS-PAGE, quality
score and chapatti.

Post-review Rundown

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