Bakshi And Bhagwat

Bakshi and Bhagwat

Greener Journal of Agricultural Sciences Vol. 6 (11), pp. 349-355, December 2016.

 ISSN: 2276-7770 

Research Articles

Manuscript Number: 120816211


(DOI: http://doi.org/10.15580/GJAS.2016.11.120816211)

 

High Moleular Weight Glutenin Subunit Variation among Indian
Wheat Varieties

 

Bakshi Suman* and Bhagwat SG

 

Nuclear Agriculture
and Biotechnology Division, Bhabha Atomic Research Centre, Trombay,
Mumbai-400085, India.


Abstract

Improving
wheat for end use quality is an important goal for wheat breeders. The
composition of high molecular weight glutenin subunits (HMW-GS) contributes to
a great extent in determining wheat quality for processing. The HMW-GS are
known to be encoded by six genes located at the Glu-1 loci on the long arms of chromosomes 1A, 1B and 1D. The
endosperm storage proteins of 62 Indian bread wheat cultivars were fractionated
by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) to
determine their high molecular weight (HMW) glutenin subunit composition. In
total, three alleles (Ax2*, Ax1 or Ax null) at Glu-A1, seven allele/allelic pairs (Bx7, Bx7+By8, Bx7+By9, Bx20,
Bx13+By16, Bx13+By19 and Bx17+By18) at Glu-B1
and two allelic pairs (Dx2+Dy12 and Dx5+Dy10) at Glu-D1 were identified in the
analysed varieties. Allele or allelic pair Ax2* (59.7%) at Glu-A1, Bx17+By18 (27.4%) at Glu-B1
and Dx2+Dy12 (69.4%) at Glu-D1 were
present most frequently in wheat
varieties taken
for study.

                Quality
score of varieties were ranged from 4 to 10, with an average of 7.55 indicating
vast genetic diversity among the varieties. The presence of subunits
combinations
in different varieties with low, medium and high gluten
strength indicated that breeding wheat for bread, chapatti and biscuits is
possible as per end use requirement.

 

Key words: Bread wheat, SDS-PAGE, quality
score and chapatti.

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References

Bhagwat SG and Bhatia CR (1993).
Selection for quality in early generation based on the HMW subunits of glutenin
in bread wheat. Indian J. Genet. 53: 208-214.

 

Branlard G and Dardevet M (1985). Diversity of grain protein and
bread wheat quality. II. Correlation between high molecular subunits of
glutenin and flour quality characteristics
. J. Cereal Sci. 3: 345-354.

 

Fang J, Liu Y, Luo J, Wang Y,
Shewry PR and He G (2009). Allelic variation and genetic diversity of high
molecular weight glutenin subunit in Chinese endemic wheats (Triticum aestivum L.). Euphytica
166:177-182.

 

Gianibelli M, Gupta RB, Lafiandra
D, Margiotta B and MacRitchie F (2001). Polymorphism of high Mr glutenin
subunits in
Triticum
tauschii
:
characterization by chromatography and electrophoretic methods.
J. Cereal Sci. 33:39-52.

 

Gupta RB, Paul JG, Cornish GB,
Palmer GA, Bekes F and Rathjen AJ (1994). Allelic variation at glutenin subunit
and gliadin loci, Glu-1, Glu-3 and Gli-1 of common wheats. I. Its additive and
interaction effects on dough properties.
J. Cereal Sci. 19: 9-17.

 

Harberd NP, Bartels D and
Thompson RD (1986). DNA restriction fragment variation in the gene family
encoding high molecular weight (HMW) glutenin subunits of wheat. Biochem.Genet.
24: 579-596.

 

 He ZH, Liu L, Xia XC, Liu JJ and Pena RJ
(2005). Composition of HMW and LMW glutenin subunits and their effects on dough
properties, pan bread, and noodle quality of Chinese bread wheats. Cereal Chem.
82: 345-350

 

Jondiko TO, Alviola NJ, Hays DB,
Ibrahim A, Tilley M and Awika, JM (2012). Effect of high-molecular-weight
glutenin subunit allelic composition on wheat flour tortilla quality. Cereal
Chem. 89:155-161.

 

Laemmli UK (1970). Cleavage of
structural proteins during assembly of the head of bacterio-phage T4. Nature
227: 680-685.

 

Li Y, Huang Ch, Sui X, Fan Q, Li
G and Chu X (2009). Genetic variation of wheat glutenin subunits between
landraces and varieties and their contributions to wheat quality improvement in
China. Euphytica 169: 159-168.

 

Liu Y, Xiong ZY, He YG, Shewry PR
and He GY (2007). Genetic diversity of HMW glutenin subunit in Chinese common
wheat (Triticum aestivum L.)
landraces from Hubei province. Genet. Resour. Crop Evol.54: 865-874.

 

Mondal S, Tilley M, Alviola JN,
Waniska RD, Bean SR, Glover KD and Hays DB (2008). Use of near-isogenic wheat lines
to determine the glutenin composition and functionality requirements for flour
tortillas. J. Agric. Food Chem.56:179-184.

 

Ng PKW and Bushuk W (1988).
Statistical relationship between high molecular weight subunits of glutenin and
bread making quality of Canadian grown wheats. Cereal Chem.65: 408-413.

 

Payne PI, Nightingale MA,
Krattiger AF and Holt LM (1987). The relationship between HMW glutenin subunit
composition and the bread-making quality of British-grown wheat varieties. J.
Sci. Food Agric. 40: 51-65.

 

Payne PI (1987). Genetics of
wheat storage proteins and the effect of allelic variation on bread making
quality. Ann. Rev. Plant Physiol. 8: 141-153.

 

Payne PI and Lawrence GJ (1983).
Catalogue of alleles for complex gene loci, Glu-A1,
Glu-B1 and Glu-D1 which code for high molecular weight subunits of glutenin in
hexaploid wheat. Cereal Res. Commun. 11: 29-35.

 

Pogna NE and Mellini F (1986).
Alla ricerca delle basi biochemiche e genetiche della qualita del
glutine.L’Inform Agr 42: 65-66.

 

Pogna NE, Mellini F, Beretta A
and Deruffo A (1989). The high-molecular-weight glutenin subunits of common
wheat cultivars grown in Italy. J. Genet. Breed. 43: 17-24.

 

Pogna NE, Radaelli R, Dackevitch
T, Curioni A,  Dal Belin Perufo A (1992).
Benefits from genetics and molecular biology to improve the end use properties
of cereals. In: Feillet P (ed) Cereal chemistry and technology: a long past and
a bright future, Montpellier, France, INRA, 83-93.

 

Ram S (2003). High Molecular
Weight Glutenin Subunit Composition of Indian Wheats and their Relationships
with Dough Strength. J. Plant Biochem. Biot.12:151-155.

 

Sreeramulu G, Banerjee R,
Bharadwaj A and Vaishnav PP (2004). Understanding the functionality of Wheat
High Molecular Weight Glutenin Subunits (HMW‐GS) in Chapati Making Quality. In:
The Gluten Proteins; The Royal Society of Chemistry, 162-165.

 

Tanaka H, Tomita M, Tsujimoto H,
YasumuroY (2003). Limited but specific variations of seed storage proteins in
Japanese common wheat (Triticum aestivum L.).
Euphytica 132:167-174.

 

Uthayakumaran S, Lukow OM, Jordan
MC and Cloutier S (2003). Development of genetically modified wheat to assess
its dough functional properties. Mol. Breed. 11:249-258.

 

Zhang P, Jondiko TO, Tilley M and
Awika JM (2014). Effect of high molecular weight glutenin subunit composition
in common wheat on dough properties and steamed bread quality. J. Sci. Food
Agr. 94: 2801-2806.

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