Bakshi and Bhagwat Greener Journal of Agricultural Sciences Vol. 6 (11), pp. 349-355, December 2016. ISSN: 2276-7770 Research Articles Manuscript Number: 120816211 (DOI: http://doi.org/10.15580/GJAS.2016.11.120816211) High Moleular Weight Glutenin Subunit Variation among Indian Wheat Varieties Bakshi Suman* and Bhagwat SG Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, Trombay, Mumbai-400085, India. Abstract Improving wheat for end use quality is an important goal for wheat breeders. The composition of high molecular weight glutenin subunits (HMW-GS) contributes to a great extent in determining wheat quality for processing. The HMW-GS are known to be encoded by six genes located at the Glu-1 loci on the long arms of chromosomes 1A, 1B and 1D. The endosperm storage proteins of 62 Indian bread wheat cultivars were fractionated by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) to determine their high molecular weight (HMW) glutenin subunit composition. In total, three alleles (Ax2*, Ax1 or Ax null) at Glu-A1, seven allele/allelic pairs (Bx7, Bx7+By8, Bx7+By9, Bx20, Bx13+By16, Bx13+By19 and Bx17+By18) at Glu-B1 and two allelic pairs (Dx2+Dy12 and Dx5+Dy10) at Glu-D1 were identified in the analysed varieties. Allele or allelic pair Ax2* (59.7%) at Glu-A1, Bx17+By18 (27.4%) at Glu-B1 and Dx2+Dy12 (69.4%) at Glu-D1 were present most frequently in wheat varieties taken for study. Quality score of varieties were ranged from 4 to 10, with an average of 7.55 indicating vast genetic diversity among the varieties. The presence of subunits combinations in different varieties with low, medium and high gluten strength indicated that breeding wheat for bread, chapatti and biscuits is possible as per end use requirement. Key words: Bread wheat, SDS-PAGE, quality score and chapatti. Return to Content View [Full Article – PDF] [Full Article – HTML] [Full Article – EPUB] Post-review Rundown View/get involved, click [Post-Review Page] References Bhagwat SG and Bhatia CR (1993). Selection for quality in early generation based on the HMW subunits of glutenin in bread wheat. Indian J. Genet. 53: 208-214. Branlard G and Dardevet M (1985). Diversity of grain protein and bread wheat quality. II. Correlation between high molecular subunits of glutenin and flour quality characteristics. J. Cereal Sci. 3: 345-354. Fang J, Liu Y, Luo J, Wang Y, Shewry PR and He G (2009). Allelic variation and genetic diversity of high molecular weight glutenin subunit in Chinese endemic wheats (Triticum aestivum L.). Euphytica 166:177-182. Gianibelli M, Gupta RB, Lafiandra D, Margiotta B and MacRitchie F (2001). Polymorphism of high Mr glutenin subunits in Triticum tauschii: characterization by chromatography and electrophoretic methods. J. Cereal Sci. 33:39-52. Gupta RB, Paul JG, Cornish GB, Palmer GA, Bekes F and Rathjen AJ (1994). Allelic variation at glutenin subunit and gliadin loci, Glu-1, Glu-3 and Gli-1 of common wheats. I. Its additive and interaction effects on dough properties. J. Cereal Sci. 19: 9-17. Harberd NP, Bartels D and Thompson RD (1986). DNA restriction fragment variation in the gene family encoding high molecular weight (HMW) glutenin subunits of wheat. Biochem.Genet. 24: 579-596. He ZH, Liu L, Xia XC, Liu JJ and Pena RJ (2005). Composition of HMW and LMW glutenin subunits and their effects on dough properties, pan bread, and noodle quality of Chinese bread wheats. Cereal Chem. 82: 345-350 Jondiko TO, Alviola NJ, Hays DB, Ibrahim A, Tilley M and Awika, JM (2012). Effect of high-molecular-weight glutenin subunit allelic composition on wheat flour tortilla quality. Cereal Chem. 89:155-161. Laemmli UK (1970). Cleavage of structural proteins during assembly of the head of bacterio-phage T4. Nature 227: 680-685. Li Y, Huang Ch, Sui X, Fan Q, Li G and Chu X (2009). Genetic variation of wheat glutenin subunits between landraces and varieties and their contributions to wheat quality improvement in China. Euphytica 169: 159-168. Liu Y, Xiong ZY, He YG, Shewry PR and He GY (2007). Genetic diversity of HMW glutenin subunit in Chinese common wheat (Triticum aestivum L.) landraces from Hubei province. Genet. Resour. Crop Evol.54: 865-874. Mondal S, Tilley M, Alviola JN, Waniska RD, Bean SR, Glover KD and Hays DB (2008). Use of near-isogenic wheat lines to determine the glutenin composition and functionality requirements for flour tortillas. J. Agric. Food Chem.56:179-184. Ng PKW and Bushuk W (1988). Statistical relationship between high molecular weight subunits of glutenin and bread making quality of Canadian grown wheats. Cereal Chem.65: 408-413. Payne PI, Nightingale MA, Krattiger AF and Holt LM (1987). The relationship between HMW glutenin subunit composition and the bread-making quality of British-grown wheat varieties. J. Sci. Food Agric. 40: 51-65. Payne PI (1987). Genetics of wheat storage proteins and the effect of allelic variation on bread making quality. Ann. Rev. Plant Physiol. 8: 141-153. Payne PI and Lawrence GJ (1983). Catalogue of alleles for complex gene loci, Glu-A1, Glu-B1 and Glu-D1 which code for high molecular weight subunits of glutenin in hexaploid wheat. Cereal Res. Commun. 11: 29-35. Pogna NE and Mellini F (1986). Alla ricerca delle basi biochemiche e genetiche della qualita del glutine.L’Inform Agr 42: 65-66. Pogna NE, Mellini F, Beretta A and Deruffo A (1989). The high-molecular-weight glutenin subunits of common wheat cultivars grown in Italy. J. Genet. Breed. 43: 17-24. Pogna NE, Radaelli R, Dackevitch T, Curioni A, Dal Belin Perufo A (1992). Benefits from genetics and molecular biology to improve the end use properties of cereals. In: Feillet P (ed) Cereal chemistry and technology: a long past and a bright future, Montpellier, France, INRA, 83-93. Ram S (2003). High Molecular Weight Glutenin Subunit Composition of Indian Wheats and their Relationships with Dough Strength. J. Plant Biochem. Biot.12:151-155. Sreeramulu G, Banerjee R, Bharadwaj A and Vaishnav PP (2004). Understanding the functionality of Wheat High Molecular Weight Glutenin Subunits (HMW‐GS) in Chapati Making Quality. In: The Gluten Proteins; The Royal Society of Chemistry, 162-165. Tanaka H, Tomita M, Tsujimoto H, YasumuroY (2003). Limited but specific variations of seed storage proteins in Japanese common wheat (Triticum aestivum L.). Euphytica 132:167-174. Uthayakumaran S, Lukow OM, Jordan MC and Cloutier S (2003). Development of genetically modified wheat to assess its dough functional properties. Mol. Breed. 11:249-258. Zhang P, Jondiko TO, Tilley M and Awika JM (2014). Effect of high molecular weight glutenin subunit composition in common wheat on dough properties and steamed bread quality. J. Sci. Food Agr. 94: 2801-2806.