Meleta And Abera


Meleta and Abera

Greener Journal of Agricultural Sciences

Vol. 9(4), pp. 436-446, 2019

ISSN: 2276-7770

Copyright ©2019, the copyright of this article is retained by the author(s)

DOI Link:







Effects of
Rhizobium Inoculation and Phosphorus Fertilizer rates on Nitrogen Fixation and
Nutrient up take of Chickpea (Cicer
L.) at Goro, Bale Zone, Oromia Regional State.



Tamiru Meleta 1*; Girma Abera 2


 1 Sinana
Agricultural Research Centre, Bale-Robe, Ethiopia

2 Hawassa University
College of Agriculture, Hawassa






Article No.: 101419186

Type: Research

DOI: 10.15580/GJAS.2019.4.101419186



Chickpea response to two varieties of chickpea (Arerti and
Habr), two Rhizobial inoculants (EAL 018 and EAL 029) and five P rate (0, 15,
30, 45 and 60 kg P2O5 ha-1) were studied in split plot arrangements. Main
plot consisted of varieties whereas P2O5 levels and Rhizobium strains were
kept in subplot. The experiment was laid out at Goro, Bale Zone, Oromia
Regional State  during 2015/16  with the objective to determine the
effectiveness of Rhizobium strains and phosphorus fertilizer application on
two varieties of chickpea (Arerti and Habru).The nodulation rating, nodule
volume and color were not significantly affected due to varieties. However,
significant variation (P <0.05) was observed in number of nodule per plant
(NNPP) and nodule dry weight (NDW) between varieties of chick pea, maximum
NNPP and NDW was obtained from Arerti variety. All the nodulation parameters
were significantly (P<0.05) affected due to rhizobium inoculation and P2O5
except the nodule color. Maximum nodulation parameters were recorded at 45
and 60 kg P2O5 ha-1and inoculation by EAL 029 strain except the nodule color.
Significant interaction effect was observed between variety and Rhizobium
inoculation for nodule rate (NR), NDW between variety and P2O5, rhizobium and
P2O5 and also interaction effect between varieties, Rhizobium and P2O5 on NR
and NDW. The N and P uptake at mid flowering, %Ndfa, grain, straw and total N
and P content and crude protein content were significantly affected by
variety, rhizobium inoculation and application of P2O5. Maximum response was
obtained from EAL 029 strain inoculations and application of 45 kg P2O5 ha-1
for N uptake, seed N and P uptake were maximum at 45and 60 kg P2O5 ha-1
respectively. The current investigation indicated that Arerti variety
inoculation with Rhizobium strain EAL 029 along with application of P2O5 at
rate of 45 kg ha-1 found to be appropriate for chickpea production in the
study area. The current investigation indicated that the use of P2O5 with
Rhizobium inoculation as a nutrient management strategy could increase chick
pea production. Since the experiment was conducted only for one year, we
suggest the results to be verified with more varieties of chickpea and
rhizobium strain under the same agro-climatic conditions.


Submitted: 14/10/2019

Accepted:  29/10/2019

Published: 01/11/2019


*Corresponding Author

Tamiru Meleta

E-mail: tamish09@ gmail. com


Keywords: Phosphorus; Rhizobia strain; Nutrient
uptake; Chickpea; Bale Robe.



Return to Content       View [Full Article – PDF]  

[Full Article – HTML]               [Full Article – EPUB]

Post-Publication Peer-review Rundown

View/get involved, click [Peer-review]




Desa. 2009. Effect of phosphorus application and rhizobium inoculation on nodulation, yield and yield related
traits of fenugreek (Trigonella foenum-graecum L.) in
sinana, south eastern Ethiopia. Thesis, Haramaya University, Haramaya,


M.E., Khanam D. M., Bhuiya H. A., Khatun M.R. and Talukder M.R. 2008. Effect of
Rhizobium inoculation on different varieties of garden pea (Pisum
L.). J. Soi. Nature2:30-33.


Azma F. 2006. Added nitrogen, it is occurrence mechanism and
implications to fate of N in the soil plant system .Pak.J.Agron.1:54-59.


K., Ali S., USAir A. 2011. Effect of different phosphorus levels on xylem sap
components and their correlation with growth variables of mash bean. Sarhad
Journal of Agriculture, Vol. 27, and No. 4.


T.J. and Evers G. 2004. Inoculation, nodulation, nitrogen fixation and
transfer. Texas cooperative extension (press).


(Central Statistics Authority). 2015. Agricultural Sample Survey. Reports on
area under cultivation, yield and production of major crops for main (Maher)
season, Ethiopia.


I. M. and Aliyu L. 2011. Yield and economic returns of sesame (Sesamumindicum L.) as influenced by
poultry manure, nitrogen and phosphorus at Samaru,  Nigeria. Elixir Agric., 39: 4884-4887.


J.L., Beaton J.D., Tisdale S.L. and Nelson W.L. 1999. Soil Fertility and
An Introduction to Nutrient Management, Prentice Hall, New
Jersey. pp 499.


M.D., Roman M.M. & Fujita M. 2007. Comparative investigation of glutathione
S-transferees, glyoxalase and alliance activities in different vegetable crops.
Journal of Crop Science and Biotechnology. 10, 21-28, ISSN 2005-8276.


J. 2004. Forage legume inoculation. In: Agriculture and Natural Resources
University of Arkansa press. UK.


G., Berkeley E., Imia M. 2012. Genetic diversity and population structure of
Ethiopian chickpea (Cicero arietinum
L.) germplasm accessions from different geographical origins
as revealed by microsatellite markers. Plant Mol. Biol. Rep. 30: 654 – 665.


L., Jiang H., Tian J., Zhaod J. and Liao H. 2011. Rhizobia enhance acquisition
of phosphorus from different sources by soybean plants. Plant Soil 349:25–36.


R.J. and Berger J.D. 2007. History and origin of chick pea .In:Yadaw et
al.,(eds) chick pea breeding and management pp 1.13.CABI Walling ford, UK.


S.K., Tiwari P.K. and Ttripathi R.S. 1999. Uptake and balance sheet of nitrogen
and phosphorus in gram as influenced by phosphorus, biofertilizers and
micronutrients under rain fed condition. Indian J. Agron., 44(4): 768-772.


Inst.Inc. 2002. SAS online doc. 9. Cary, In. NC: SAS Institute Inc. USA.


A., Baoule A., Ahmed HG., Dikko AU., Aliyu U.,Sokoto MB., Hassan J., Musa M.,
Haliru B. 2011. Influence of phosphorus on the performance of cowpea (Vigna unguiculata L. Walp.) varieties in
the Sudan savanna of Nigeria. Agri. Science. 2(3): 313-317.


M., Javed M. R., Tanver A., Nadem M. A. andWasaya A., Bukhari S.A.H. and Rehman
J. U. 2009. Efect of different herbicides on weeds, growth and yield of spring
planted maize (Zea mays L.). Pak. J.
Life Soc. Sci. 7(2): 168-174.


Cite this Article: Meleta, T; Abera, G (2019). Effects of Rhizobium
Inoculation and Phosphorus Fertilizer rates on Nitrogen Fixation and Nutrient
up take of Chickpea (Cicer arietinum
L.) at Goro, Bale Zone, Oromia Regional State. Greener Journal of Agricultural Sciences 9(4): 436-446, 



Leave a Reply

Your email address will not be published. Required fields are marked *