Greener Journal of Agricultural Sciences Vol. 6 (10), pp. 304-311, November 2016.

 ISSN: 2276-7770 

Research Paper

Manuscript Number: 101916186

(DOI: http://doi.org/10.15580/GJAS.2016.10.101916186)


Aflatoxin and Fumonisin
Contamination of at-harvest and Storage Beans in Babati District, Northern


Chacha Nyangi


Department of Science and Business Management, Mbeya University of
Science and Technology, P.O. Box 131, Mbeya, Tanzania.


The natural occurrence of total aflatoxin
and fumonisin was determined in 38 bean at-harvest samples and 106 storage
beans samples in the year 2013 in Babati District Northern Tanzania.
Quantification for total aflatoxin and fumonisin was done using Enzymes Linked
Immunosorbent Assay – ELISA (Reveal AccuScan® Neogen, USA), and the
results were confirmed using Liquid Chromatography Tandem Mass Spectrometer
(LC-MS/MS). Eighteen percent (7/38) of bean at harvest samples were
contaminated with up to 3 µg/kg aflatoxins and no bean sample was contaminated
with fumonisins. There was a significant correlation (p < 0.05) between the
concentration of aflatoxins and fumonisins with climatic zones and agronomic
practices. Only bean samples from the high altitude high rain zone were
contaminated with aflatoxins (mean level of 1.53 µg/kg).  For storage beans, samples from Seloto
village were associated with higher aflatoxin concentration (mean of 3.74
µg/kg) and those from Long village were associated with higher fumonisin levels
(mean of 9.0 mg/kg). These results indicate that beans consumers in the study
area are exposed to the danger of chronic exposure to aflatoxin and fumonisin
poisoning. Thus, those practices that reduce contamination should be adopted by
all farmers in the study area to reduce the health hazards associated with
consumption of contaminated beans. This also calls for further research to
investigate human exposure to aflatoxin and fumonisin in the study area.


Keywords: Aflatoxin, fumonisin,
at-harvest, storage practices, beans, 
Babati, Tanzania.

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