Tizhe et al Greener Journal of Agricultural Sciences Vol. 9(3), pp. 309-314, 2019 ISSN: 2276-7770 Copyright ©2019, the copyright of this article is retained by the author(s) DOI Link: https://doi.org/10.15580/GJAS.2019.3.072719146 https://gjournals.org/GJAS Fungal Pathogens Associated with Watermelon (Citrullus lanatus) Fruit and Efficacy Determination of Annona senegalensis Leaf Extract Against The Fungal Isolates 1Tizhe Tari Dlama*, 1Yusuf S. Comfort, 1Zakawa Ngida Ndale, 2Dagze John Kagana and 1Emmanuel Abigail 1Department of Botany, Adamawa State University, Mubi, Nigeria 2Department of Laboratory Science, Federal Polytechnic, Mubi, Nigeria ARTICLE INFO ABSTRACT Article No.: 072719146 Type: Research DOI: 10.15580/GJAS.2019.3.072719146 This study focused on the Isolation of fruit-rot fungal pathogens of watermelon (Citrullus lanatus) and the efficacy determination of Annona senegalensis leaf extract against the fungal isolates. A total of ten (10), each of the spoilt and healthy watermelon fruit from among the samples collected from the four different markets within Mubi were randomly selected for the isolation of fruit-rot fungal pathogens. The collected A. senegalensis fresh leaves were air-dried, pulverized and extracted using maceration method of extraction. The extract was then screened for the presence of phytochemicals and antifungal activity test. The study indicated the presence of only two fungal species such as Aspergillus fumigatus and Aspergillus niger associated with the fruit-rot of watermelon. Terpenoids, flavonoids and tannins were the phytochemicals discovered present in the ethanolic leaf extract of A. senegalensis. The antifungal activity test of the extract showed an inhibitory effect against all the fungal isolates with statistically highest diameter zone of inhibition of 17.50 mm and 20.85 mm and lowest zone of 7.80 mm and 3.15 mm on A. fumigatus and A. niger respectively. Fungal species such as A. niger and A. fumigatus were responsible for the fruit-rot of watermelon fruit obtained from Mubi region. The ethanolic leaf extract of A. senegalensis was effective against A. fumigatus and A. niger. Submitted: 27/07/2019 Accepted: 30/07/2019 Published: 26/08/2019 *Corresponding Author Tizhe Tari Dlama E-mail: taritizhe@ yahoo. com Phone: +2347082547204 Keywords: Fungi; Antifungal activity; Isolation; watermelon Return to Content View [Full Article – PDF] [Full Article – HTML] [Full Article – EPUB] Post-Publication Peer-review Rundown View/get involved, click [Peer-review] REFERENCES Adebayo AA (2004). Mubi Region Geographic Synthesis, first edition paracelet publishers, Yola, Nigeria, p 19. Adekunle AA, Fatunbi AO, Adisa S, Adeyemi OA (2007). Growing watermelon commercially in Nigeria: An illustrated guide. USAID ICS Nigeria. Afsah-Hejri L, Jinap S, Hajeb P, Radu S, Shakibazadeh S (2013). A review on mycotoxins in food and feed: Malaysia case study. 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