Chukunda And Onyeizu

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Chukunda and Onyeizu

Greener
Journal of Agricultural Sciences

Vol.
9(1), pp. 113-118, 2019

ISSN:
2276-7770

Copyright
©2019, the copyright of this article is retained by the author(s)

DOI
Link:
http://doi.org/10.15580/GJAS.2019.1.031419046

http://gjournals.org/GJAS

 

 

Influence
of culture media, temperature and light/darkness on the mycelial growth of Lasiodiplodia theobromae (Pat.)

 

1Chukunda, F.A.; 2Onyeizu, U.R.

 

1Department of Forestry and
Environment, Faculty of Agriculture, Rivers State University of Science and
Technology, Port Harcourt, Nigeria.

2Department of Environmental
Management & Toxicology, Michael Okpara University of Agriculture, Umudike,
Abia State, Nigeria

 

ARTICLE INFO

ABSTRACT

 

Article
No.:
031419046

Type: Research

DOI: 10.15580/GJAS.2019.1.031419046

 

 

In this study, the effects of culture media, temperature and light
and darkness on mycelial growth of L. theobromae were evaluated. Results of
potato dextrose agar (PDA) and potato dextrose agar stem exudates (PDASE)
were found suitable for mycelial growth of the L. theobromae. The fungus
grew from 20-40oC with optimum growth observed at 25-35oC on both media. On
the 10th day, mycelial growth at 25-35oC was (15.6mm ± 0.02 – 30.6mm ± 0.05;
18.4mm ± 0.28 – 32.5mm ± 0.10) while mycelial growth recorded with PDASE was
(18.2mm ± 0.23 – 31.0mm ± 0.25; 22.8mm ± 0.02 – 38.2mm ± 0.40). There were
no significant effects of light and darkness on the mycelial difference (P≤
0.05) on the growth of L. theobromae. It is recommended from this research
work that amended potato dextrose agar (PDA) with stem exudates promoted the
growth of test fungus L. theobromae and hence should be used as culture
medium for fungi at 35oC.

 

Submitted:
14/03/2019

Accepted:  21/03/2019

Published: 30/03/2019

 

*Corresponding Author

Chukunda, F.A.

E-mail:
onyifrank2002@ yahoo.com

Phone: 08037501179

 

Keywords: Culture
media, Potato dextrose agar stem exudates (PDASE), potato dextrose agar
(PDA)

.

 

 

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Cite this Article: Chukunda, FA; Onyeizu, UR (2019). Influence of
culture media, temperature and light/darkness on the mycelial growth of Lasiodiplodia theobromae (Pat.).
Greener Journal of Agricultural Sciences 9(1): 113-118,
http://doi.org/10.15580/GJAS.2019.1.031419046.


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