Chukunda and Onyeizu Greener Journal of Agricultural Sciences Vol. 9(1), pp. 113-118, 2019 ISSN: 2276-7770 Copyright ©2019, the copyright of this article is retained by the author(s) DOI Link: http://doi.org/10.15580/GJAS.2019.1.031419046 http://gjournals.org/GJAS Influence of culture media, temperature and light/darkness on the mycelial growth of Lasiodiplodia theobromae (Pat.) 1Chukunda, F.A.; 2Onyeizu, U.R. 1Department of Forestry and Environment, Faculty of Agriculture, Rivers State University of Science and Technology, Port Harcourt, Nigeria. 2Department of Environmental Management & Toxicology, Michael Okpara University of Agriculture, Umudike, Abia State, Nigeria ARTICLE INFO ABSTRACT Article No.: 031419046 Type: Research DOI: 10.15580/GJAS.2019.1.031419046 In this study, the effects of culture media, temperature and light and darkness on mycelial growth of L. theobromae were evaluated. Results of potato dextrose agar (PDA) and potato dextrose agar stem exudates (PDASE) were found suitable for mycelial growth of the L. theobromae. The fungus grew from 20-40oC with optimum growth observed at 25-35oC on both media. On the 10th day, mycelial growth at 25-35oC was (15.6mm ± 0.02 – 30.6mm ± 0.05; 18.4mm ± 0.28 – 32.5mm ± 0.10) while mycelial growth recorded with PDASE was (18.2mm ± 0.23 – 31.0mm ± 0.25; 22.8mm ± 0.02 – 38.2mm ± 0.40). There were no significant effects of light and darkness on the mycelial difference (P≤ 0.05) on the growth of L. theobromae. It is recommended from this research work that amended potato dextrose agar (PDA) with stem exudates promoted the growth of test fungus L. theobromae and hence should be used as culture medium for fungi at 35oC. Submitted: 14/03/2019 Accepted: 21/03/2019 Published: 30/03/2019 *Corresponding Author Chukunda, F.A. E-mail: onyifrank2002@ yahoo.com Phone: 08037501179 Keywords: Culture media, Potato dextrose agar stem exudates (PDASE), potato dextrose agar (PDA) . Return to Content View [Full Article – PDF] [Full Article – HTML] [Full Article – EPUB] Post-Publication Peer-review Rundown View/get involved, click [Peer-review] RFERENCES Alam, S.M., Most-Ferdousi, B., Montaz, A.S., Rafiqul Islam, M. & Shah-Alam-M (2001). Effect of Temperature, Light and Media on growth, sporulation, formation of pigments and Pycnidia of Botryodiplodia theobromae Pat. Pakistan Journal of Biological Science, 4 (10): 1224-1227. Chukunda, F.A (2014). Post-harvest fungal diseases of Persea gratissima (C.F. Gaerth) and Dacryodes edulis (G.Don) H.J. Lam, Fruits. Rivers State, University of Science and Technology, Ph.D Rivers State, Nigeria, 21. Cochrane, V. W (1958). Physiology of fungal. John Wiley & Sons, Inc. New York, 524. Crammer, J (1979). Botryodipiodia theobromae. Commonwealth Mycological Institute, Kew, Surrey. 123. Domsch, K.H, Gams, W. & Anderson, T.H. (2007). Compendium of Soil Fungi. 2 (ed). Cornell University, England. French, B. R (2006). Diseases of food plants in Papua New Guinea– A compendium. 38. Jash, S., Dutta, S. Bandyopadhyay S. &. Laha (2003). Effect of different culture media, pH and carbon sources on growth and sporulation of Anemaria zinniae Pape causing leaf and flower blight of marigold. Environmental Ecology, 21, 321-325. Jiskani M.M. (2002). Dying of mango orchards needs special attention, Sindh Agriculture University, Pakistan. Kartatti, R. S. & Hiremath, P. C. (1989). Cultural and growth characteristics of Alternada zinniae, causal agent of leaf and inflorescence blight of mangold. Mysore Journal Agricultural Science, 23, 487489. Khanzada, M. A., Rajput, Q.A & Shahzad S (2006). Effect of medium, temperature, light and inorganic fertilizers on In vitro growth and sporulation of Lasiodiplodia theobromae isolated from mango. Pakistan Journal of Science, 38(3):885-889. Khanzada, M.A, Lodhi, A.M & Shahnd S. (2004a). Mango dieback and gummosis in Sindh. Pakistan caused by Lasiodiplodia theobromae. Plant Health Progress. Khanzada, M.A, Lodhi, A.M & Shahnd S. (2004b). Pathogenicity of Lasiodiplodia theobromae and Fusatium solani on mango. Pakistan Journal of Science, 36(1):181-189. Kuhn, D. D. & Ghonnoum, M. A (2003). Indoor mold, toxigenic fungi, and starchy Botrys chartarum: Infectious disease perspective. Clinical Microbiology Review, 16 (1): 144-172. Kumar P.S. and Singh S.R (2000). First report of Lasiodiplodia thecbromae as a foliar pathogen of Padhenkni hysterophorus. Plant Disease, 84, 1343. Kumara K. L W & Rawal R. D (2008). Influence of carbon, nitrogen, temperature and pH on the growth and sporulation of some Indian isolates of Colletotrichum gloeosporioides causing anthracnose disease of papaya (Carrica papaya L.) Tropical Agricultural Research Extension, 11:7-12. Maheswari, S. K., Singh D. V. & Sahu A.K. (1999). Effect of several nutrient media, pH and carbon sources on growth and sporulation of Altemaria altemata. Journal of Mycopathology Research, 37, 21-23. Mohali, S. Burgess, T.I & Winglleld, M. J. (2005). Diversity and host association of the tropical Tree endophyte Lasiodiplodia theobromae revealing using simple sequence repeat markers, Berlin Forest Pathology, 35:385-396. Oladiran, A. O. & Iwu, L. N (1993). Studies on fungi associated with tomato fruit rots and effect of environmental factors on storage. Mycologia, 21:157-163. Onyenka, T.J, Dixon, A.G.O & Ekpo, E.J.A. (2005). Identification of levels of resistance to cassava root rot disease (Bohyaiipmdia theobromae) in Africa landrace and improved germplasm using in-vitro inoculation methods. Euphytica 1457(3), Netherlands, Springer. 281-288. Phillips A.J.L (2007). Lasiodiplodia theobromae Portugal Centro de Recursos Microbiobgicos, Faculdade de Ciênciase Tecnologia, Universidade Nova de Lisboa, 2. Rawal, N. & Grewel, J. S (1989). Effect of temperature, light and relative humidity on conidial germination of three stains of Botrytis cinerea infecting chickpea Indian Phytopathology 42: 79-83. Ray, R. C (2004). Extracellular amylase (production by fungi) Botryodipiodia theobromae and Rhizopus oryzae grown on cassava starch residue. Journal of Environmental Biology, 25, 489495. Rieger M (2006). Introduction to fruit crops. The Haworth Press. NewYork, 87. Saleem A & Nasir, M. A. (1991). Culture Media Directorate of Agricultural Information, Agriculture Department, Government of the Punjab, Lahore. Sangchote, S (1988). Botryodiplodia stem end rot of mango and its control. Kasetsart Journal of Natural Science Supply, 22:67-70. Sangeetha, G. Anandan A, Rani S. U (2011). Morphological and Molecular characterization of Lasiodiplodia theobromae from various banana cultivars causing crown rot disease in fruits. Archives of Phytopathology and Plant protection, 1-12. Schwemmin, D. J (1960). Light controlled reproductive differentiation in Aspergillus ornatus. University of Michigan, U.S.A. Steel, R. G. D & Torrie J. H. (1980). Principles and Procedures of Statistics: A Biometrical Approach. 2nd edition. 597. McGraw Hill Book Company, New York. Sutton, B.C. (1980). The Coelomycetes Common wealth Mycological Institute. Kew, Surrey. Teyegaga, A. & Clerk, G. C (1972). Germination and survival of conidia of Cercospora caneslens. Tropical Agriculture, 6: 197-204. Ukoima H. N. and Chukunda, F. A (2016). Influence of physiological factors on mycelial growth of Botryodiplodin theobromae Pat., isolated from Annona muricata. Nigerian Journal of Oil and Gas Technology, 1 (1): 39-50. Xu, S.O., Yuan, S.Z. & Chen X. C. (1984). Studies on pathogenic fungus (Allernada tennrris Nees) of poplar leaf blight. Journal of North East Forestry Institution, 12, 56-64. Cite this Article: Chukunda, FA; Onyeizu, UR (2019). Influence of culture media, temperature and light/darkness on the mycelial growth of Lasiodiplodia theobromae (Pat.). Greener Journal of Agricultural Sciences 9(1): 113-118, http://doi.org/10.15580/GJAS.2019.1.031419046.