Garuma Greener Journal of Agricultural Sciences Vol. 9(1), pp. 86-89, 2019 ISSN: 2276-7770 Copyright ©2019, the copyright of this article is retained by the author(s) DOI Link: http://doi.org/10.15580/GJAS.2019.1.012119024 http://gjournals.org/GJAS Isolation and Characterization of Mango Shoot Dieback Pathogen Garuma Nemera Roge Ethiopian Institute of Agricultural Research (EIAR) Holetta Agricultural Research Center, P.O BOX 31 ARTICLE INFO ABSTRACT Article No.: 012119024 Type: Research DOI: 10.15580/GJAS.2019.1.012119024 Mango, Mangifera indica, is an economically important tropical fruit, which is produced in many countries around the world, and this plant is affected by more than 140 different plant pathogens during its life cycle. The study was conducted to isolate and characterize causal pathogen (S) of mango shoot dieback. Samples were taken from top, middle and bottom of diseased mango canopy. The samples were cultured using PDA. Two fungal pathogen species: Colletotrichum spp. and Pestalotiopsis spp. were isolated. The isolates were characterized based on their colony growth (cm), colony color, colony shape, and colony growth orientation after their further purification. The mean length of the diameter of mycelia growth for the three representatives of all isolates, i.e. top, middle and bottom showed the isolates from middle of the crown of the mango tree exhibited relatively highest mean length (5.88 cm) diameter whereas the mean length of the diameter for the isolates from top and bottom were 4.63 cm and 5.63 cm respectively. The isolates from top showed the least mean diameter length (4.63 cm). The diameter length of isolated from top was significant to the isolates from middle and bottom. The color of colony at front side of isolates from which Colletotrichum spp. was identified showed whitish color whereas the same isolates had blackish and yellowish color at their reverse side. Pastalotiopsis spp. showed whitish and yellowish colors at their front and reverse sides respectively. Circular and irregular colony shape, and spreading and upward colony growth habit (orientation) was observed on both identified fungal pathogens. The overall results showed that mango shoot dieback can be caused by more than two pathogens with different morphological characteristics. Submitted: 21/01/2019 Accepted: 29/01/2019 Published: 10/03/2019 *Corresponding Author Garuma Nemera Roge E-mail: grmn2007@ gmail. com Phone: +(251) 9-17-88-88-69 Keywords: Mango shoot dieback; Colletotrichum spp.; Pestalotiopsis spp.; Isolates Return to Content View [Full Article – PDF] [Full Article – HTML] [Full Article – EPUB] Post-Publication Peer-review Rundown View/get involved, click [Peer-review] REFERENCES Espinoza JG, Briceño EX, Chávez ER, Úrbez-Torres JR, and Latorre A (2009). Neofusicoccum spp. associated with stem canker and dieback of blueberry in Chile. Plant Disease 93: 1187-1194. Evans EA (2008). Recent trends in world and U. S. mango production trade, and consumption. University of Florida, IFAS Extension. Haggag WM (2010). Mango diseases in Egypt. Agriculture and Biology Journal of North America 1: 285-289. Ismail AM (2012). Studies on the fungal diseases of mango with particular reference to diseases Caused by Botryosphaeria species. Doctoral Thesis, University of Catania Faculty of Agriculture, Egypt. Khanzada MA, Lodhi AM and Shahzad S (2004a). Mango dieback and gummosis in Sindh, Pakistan caused by Lasiodiplodia theobromae. Plant Health Progress Online:http://www.plantmanagementnetwork.org/pub/php/diagnosticguide/2004/mango/. Khanzada MA, Lodhi AM and Shahzad S (2004b). Pathogenicity of Lasiodiplodia theobromaeand Fusarium solani on mango. Pakistan Journal of Botany 36: 181-189. Ploetz RC (2003). Diseases of mango. In: Ploetz R.C. (ed.). Diseases of Tropical Fruit Crops, pp. 327-363. APS Press, St. Paul, MN, USA. Prakash O (2004). Diseases and disorders of mango and their management. In: Naqvi SAMH (ed.). Diseases of Fruits and Vegetables, pp. 511-619. Kluwer Academic Publishers, Dordrecht, the Netherlands. Saeed EE, Sham A, AbuZarqa A, A Al Shurafa K, S Al Naqbi T, Iratni R, El-Tarabily K, F AbuQamar S (2017). Detection and Management of Mango Dieback Disease in the United Arab Emirates. International journal of molecular sciences. 2017 Oct 20; 18(10):2086. Cite this Article: Garuma NR (2019). Isolation and Characterization of Mango Shoot Dieback Pathogen. Greener Journal of Agricultural Sciences 9(1): 86-89, http://doi.org/10.15580/GJAS.2019.1.012119024.
