Garuma

Garuma

Greener Journal of Agricultural
Sciences

Vol. 9(1), pp. 86-89, 2019

ISSN: 2276-7770

Copyright ©2019, the copyright
of this article is retained by the author(s)

DOI Link: http://doi.org/10.15580/GJAS.2019.1.012119024

http://gjournals.org/GJAS

 

Isolation
and Characterization of Mango Shoot Dieback Pathogen

 

Garuma Nemera Roge

 

Ethiopian Institute of Agricultural Research
(EIAR)

Holetta Agricultural Research Center, P.O BOX
31

 

ARTICLE INFO

ABSTRACT

 

Article
No.:
012119024

Type: Research

DOI: 10.15580/GJAS.2019.1.012119024

 

 

Mango, Mangifera indica, is an economically important
tropical fruit, which is produced in many countries around the world, and
this plant is affected by more than 140 different plant pathogens during its
life cycle. The study was conducted to isolate and characterize causal
pathogen (S) of mango shoot dieback. Samples were taken from top, middle and
bottom of diseased mango canopy. The samples were cultured using PDA. Two
fungal pathogen species: Colletotrichum
spp. and Pestalotiopsis spp. were isolated. The isolates were
characterized based on their colony growth (cm), colony color, colony shape,
and colony growth orientation after their further purification. The mean
length of the diameter of mycelia growth for the three representatives of all
isolates, i.e. top, middle and bottom showed the isolates from middle of the
crown of the mango tree exhibited relatively highest mean length (5.88 cm)
diameter whereas the mean length of the diameter for the isolates from top
and bottom were 4.63 cm and 5.63 cm respectively. The isolates from top
showed the least mean diameter length (4.63 cm). The diameter length of
isolated from top was significant to the isolates from middle and bottom. The
color of colony at front side of isolates from which Colletotrichum
spp. was identified showed whitish color
whereas the same isolates had blackish and yellowish color at their reverse
side. Pastalotiopsis
spp. showed
whitish and yellowish colors at their front and reverse sides respectively.
Circular and irregular colony shape, and spreading and upward colony growth
habit (orientation) was observed on both identified fungal pathogens. The
overall results showed that mango shoot dieback can be caused by more than
two pathogens with different morphological characteristics
.

 

Submitted: 21/01/2019

Accepted:  29/01/2019

Published: 10/03/2019

 

*Corresponding
Author

Garuma
Nemera Roge

E-mail:
grmn2007@ gmail. com

Phone:
+(251) 9-17-88-88-69

 

Keywords:

Mango
shoot dieback; Colletotrichum spp.; Pestalotiopsis spp.; Isolates

 

 


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Cite this Article: Garuma NR (2019). Isolation and Characterization
of Mango Shoot Dieback Pathogen. Greener Journal of Agricultural Sciences
9(1): 86-89, http://doi.org/10.15580/GJAS.2019.1.012119024.

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