Appiah-Kubi et al Greener Journal of Agricultural Sciences, Vol. 5 (4), pp. 132-140, July 2015. ISSN: 2276-7770 Research Paper Manuscript Number: 061715082 (DOI http://doi.org/10.15580/GJAS.2015.4.061715082) Genetic variability in Colletotrichum gloeosporioides (Penz.) isolated from cassava and yam from four agro-ecological zones of Ghana *1Appiah-Kubi, Z., 2Apetorgbor, A. K., 1Moses, E., 1Quian, M., 1Thompson, R., 1Appiah-Kubi, D. 1Abrokwa, L. 1CSIR-Crops Research Institute, P. O. Box 3785, Kumasi, Ghana 2Department of Theoretical and Applied Biology, KNUST, Kumasi, Ghana *Corresponding Author’s E-mail: zipak1@ yahoo. com Abstract Anthracnose disease caused by Colletotrichum gloeosporioides (Penz.) Penz. and Sacc. is one of the most important fungal diseases of yam and cassava. To determine the genetic variations in C. gloeosporioides from the two crops, twenty-three isolates of the pathogen obtained from anthracnose-infected cassava stem and leaves and twenty-one isolates from yam leaves and vine from four agro-ecological zones of Ghana (Forest, Transition, Guinea and Coastal Savannah) were evaluated. Six species-specific internal transcribed spacer (ITS) primers were used in pairs to identify isolates to species. Amplification of the genomic DNA using specific primers ITS1 paired with ITS4 generated PCR products of band sizes between 500 to 600 bp which compared well with published data. Also eight Random Amplified Polymorphic DNA (RAPD) markers were used to determine genetic relationships among the 44 C. gloeosporioides isolates from cassava and yam. A phylogenetic tree constructed based on polymorphic bands generated with eight random primers, using the upgma method grouped forty-three of the isolates into two main clusters. The main clusters separated C. gloeosporioides isolates from cassava from those from yam. One isolate from cassava presented profiles completely different from the two main clusters and was grouped separately. Keywords: Anthracnose, Colletotrichum gloeosporioides (Penz.), molecular variations, ITS primers, RAPD. Return to Content View [Full Article – PDF] [Full Article – HTML] [Full Article – EPUB] Post-review Rundown View/get involved, click [Post-Review Page] References Adaskaveg JE and Hartin RJ (1997). Characterization of Colletotrichum acutatum isolates causing anthracnose of almond and peach in California. Plant Pathol. 87(9):979 – 987. Jaccard P (1908). Nouvelles recherches sur la distribution florale. Bul. Soc. Vaudoise Sci. Nat. 44:223-270. Kumar GA (2014). Colletotrichum gloeosporioides: Biology, Pathogenicity and Management in India. Journal of Plant Physiology & Pathology 2(2):1-11. http://dx.doi.org/10.4172/2329-955X.1000125 Kumari SS, Adiver VI, Benagi AS and Nadaf HL (2008). Molecular variation in Phaeoisariopsis personata (Berk. and M.A. Curtis) van Arx causing late leaf spot of groundnut (Arachis hypogea L.). Karnataka J. Agric. Sci. 22(2): 336-339. Padman M and Janardhana GR (2011). Occurrence and characterization of Colletotrichum gloeosporioides isolated from Murraya koenigii. New York Science Journal. 4(8): 70-76 Smith BJ and Black LL (1990). Morphological, cultural, and pathogenic variation among Colletotrichum species isolated from strawberry. Plant Dis. 74:69-76. Stanley F, Talma K and Ezra S (1996). Characterization of Colletotrichum gloeosporioides isolates from avocado and almond fruits with molecular and pathogenicity tests. Applied and Environmental Microbiology 6:1014-1020. Sutton BC (1992). The genus Glomerella and its anamorph Colletotrichum. In: Colletotrichum: Biology, Pathology and Control. J.A. Bailey and M.J. Jeger (eds). CAB International, Wallingford, UK: 1-26. Rholf FJ (1994). NTSYS-pc, numerical taxonomy and multivariate analysis system. Exeter Software, Applied Biostatistics, N.Y. Saharan Africa. pp 432-439. Vinnere O, Fatehi J, Wright SAI and Gerhardson B (2002). The causal agent of anthracnose of Rhododendron in Sweden and Latvia. Mycol. Res.106:60 – 69. Vinod T and Benagi VI (2009). Studies on the cultural and nutritional characteristics of Colletotrichum gloeosporioides, the causal organism of papaya anthracnose. Karnataka J. Agric. Sci. 22 (4):787-789. Weising K, Nybon M, Wolf K and Meyer W (1995). DNA finger printing in plants and fungi. Pl. Gen. Res. Newsltr. 97:3-39. White T J, Bruns T, Lee S and Taylor J (1990). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: PCR Protocols. Innis, M. A., Gelfand, D. H., Sninsky, J. H., White, T. J. (eds). Academic Press, San Diego, USA pp 315-322. Xiao CL, Mackenzie SJ and Legard DE (2004). Genetic and pathogenic analyses of Colletotrichum gloeosporioides isolates from strawberry and non-cultivated hosts. Phytopathology 94: 446-53. https://www.qiagen.com/resources/molecular-biology-methods/dna/. DNA Protocols and Applications-Qiagen. Assessed 11/05/15.