Attaullah Et Al

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Attaullah et al



Greener
Journal of  Agricultural Sciences

Vol. 2 (
5),
pp. 212-219,

September

2012


 ISSN: 2276-7770 



Research

Paper

Manuscript
Number:08011229

 



Comparison of Different Techniques for the Isolation of
Rhizoctonia solani
from Infected Potato Tubers.

 


1
Attaullah1,
2Shaukat Hussain1,3Farhad
Ali1,3Amjad Khan and *2Gulzar
Khan

 


1
Khyber
Pukhtunkhwa Agriculture University Peshawar


2
North
West Institute of Plateau Biology Chinese Academy of
sciences, Xining 810001, China


3
Gomal
University KPK, Dera Ismail Khan



E-mail:


attaullah.pathology@gmail.com

 


        *Corresponding Author’s email:


gulzar_khan92 @ yahoo. com

Abstract:

The effect of
surface disinfection, temperatures, nutrients and chemicals
on sclerotial germination of Rhizoctonia solani from
potato tubers were studied on potato dextrose agar (PDA).
Germination occurred from tuber pieces bearing sclerotia
pre-treated for one min with 0.1% of mercuric chloride.
Tuber pieces pretreated with 0.5% and 1%of mercuric chloride
for various lengths of times, including 15 sec, 30 sec and
one min showed no sclerotial germination on PDA. Similarly
sclerotial germination did not occur after pretreated with
sodium hypochlorite for same concentrations and times.
Sclerotial germination was optimal at a temperature of 250 C
on PDA. Germination of sclerotia was best on PDA followed by
cornmeal agar (CMA). Minimum mycelial growth occurred on
water agar (WA) while sclerotial germination was minimal on
malt extract agar (MEA) after one week of inoculation. Tuber
pieces bearing sclerotia were also treated with 0.1 % of
mercuric chloride, sodium hypochlorite, potassium hydroxide
and ethanol for one min before inoculation but no sclerotial
germination occurred on PDA except from pieces treated with
mercuric chloride.
 
Key Words: Sclerotial Germination, Mycelial Growth,
Inoculation, PDA.

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