Journal of  Agricultural Sciences

Vol. 3 (6), pp.
492-496, June 2013.

 ISSN: 2276-7770  



Manuscript Number: 011613389


Screening of Some Fungi Associated

Maize Cob Degradation for Cellulase Activity


Bamigboye, O. O.


Department of Biology,
Emmanuel Alayande College of Education, Oyo.

bamigboyetaitoy @ yahoo. com


Fungi involved in the biodegradation of maize
cob were isolated and screened for cellulase activity using
glucose and carboxylmethyl cellulose as carbon sources.  The
effect of the carbon sources on cellulase production was

Eleven fungi species were isolated which include
oryzae, Aspergillus flavus
Mucor racemosus, Aspergillus niger, Penicillium atrovenetum,
Penicillium expansum, Botryotrichum piluliferum, Penicillium
and Penicillium restictum. Effect of
carbon source on cellulase production was also reported. The
highest reducing sugar production for all fungi isolate was
obtained on day 14 when carboxymethyl cellulose (CMC) was
used as the carbon source; while the highest reducing sugar
production was obtained on day 7 when glucose was used as
the carbon source
Aspergillus niger had the highest production of
reducing sugar (1.68mg/ml and 0.82/m) in CMC and glucose
respectively while the lowest production was recorded for

Mucor racemosus
(0.69mg/,ml and 0.01mg/ml). All
species and Penicilluim
species isolated had very high reducing sugar production in
Aspergillus flavus 1.24 mg/ml; A oryzae
P expansum,
1.60 mg/ml,
P atrovenetum 1.43mg/ml) except
Penicillium chrysonenum
and Penicillium restrictum
with 0.78mg/ml and 0.81 mg/ml, respectively.

Aspergillus and Penicillum species are good
cellulase producers especially those that are associated
with degrading maize cob. These organisms are considered
suitable to increase the nutrient of maize

cob, an agro industrial waste and consequently make them
useful for animal feed production.


Keywords: Cellulase, Fungi, Reducing Sugar,
Maize-cob, Biodegradation.

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