Bamigboye

Bamigboye



Greener
Journal of  Agricultural Sciences


Vol. 3 (6), pp.
492-496, June 2013.


 ISSN: 2276-7770  



Research

Paper

Manuscript Number: 011613389

 


Screening of Some Fungi Associated

w
ith
Maize Cob Degradation for Cellulase Activity

 


Bamigboye, O. O.

 

Department of Biology,
Emmanuel Alayande College of Education, Oyo.

Email:
bamigboyetaitoy @ yahoo. com

Abstract:

Fungi involved in the biodegradation of maize
cob were isolated and screened for cellulase activity using
glucose and carboxylmethyl cellulose as carbon sources.  The
effect of the carbon sources on cellulase production was
determined. 


Eleven fungi species were isolated which include
Rhizopus
oryzae, Aspergillus flavus
,.
Mucor racemosus, Aspergillus niger, Penicillium atrovenetum,
Penicillium expansum, Botryotrichum piluliferum, Penicillium
chrysogenum
and Penicillium restictum. Effect of
carbon source on cellulase production was also reported. The
highest reducing sugar production for all fungi isolate was
obtained on day 14 when carboxymethyl cellulose (CMC) was
used as the carbon source; while the highest reducing sugar
production was obtained on day 7 when glucose was used as
the carbon source
.
Aspergillus niger had the highest production of
reducing sugar (1.68mg/ml and 0.82/m) in CMC and glucose
respectively while the lowest production was recorded for

Mucor racemosus
(0.69mg/,ml and 0.01mg/ml). All
Aspergillus
species and Penicilluim
species isolated had very high reducing sugar production in
(CMC)
:
Aspergillus flavus 1.24 mg/ml; A oryzae
1.08mg/ml,
P expansum,
1.60 mg/ml,
P atrovenetum 1.43mg/ml) except
Penicillium chrysonenum
and Penicillium restrictum
with 0.78mg/ml and 0.81 mg/ml, respectively.

Aspergillus and Penicillum species are good
cellulase producers especially those that are associated
with degrading maize cob. These organisms are considered
suitable to increase the nutrient of maize

cob, an agro industrial waste and consequently make them
useful for animal feed production.

 

Keywords: Cellulase, Fungi, Reducing Sugar,
Maize-cob, Biodegradation.
 


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