Chennappa Et Al

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Chennappa et al



Greener
Journal of  Agricultural Sciences


Vol. 4 (4), pp.
117-129,
May 2014.


 ISSN: 2276-7770 



Research

Paper

Manuscript Number: 010314003

DOI: http://dx.doi.org/10.15580/GJAS.2014.4.010314003

 



Impact of Pesticides on PGPR Activity of Azotobacter
sp. Isolated from Pesticide Flooded Paddy Soils

 



 Chennappa G.1, Adkar-Purushothama C.R.2#
Naik M.K.3

Suraj U.4, Sreenivasa
M.Y.1*

 

1Department
of Studies in Microbiology, University of Mysore,
Manasagangotri,


Mysore- 570 006, Karnataka, India.


2
Laboratory
of Plant Pathology
,


Faculty of Agriculture and Life Science


Hirosaki University, Bunkyo-cho 3, Hirosaki 036-8561
,


Aomori Ken, Japan

#Present address:
Pavilion for Applied Cancer Research, 

Department of
Biochemistry

Faculty of Medicine
and Health sciences, Sherbrooke University 3201, Rue
Jean-Mignault, Sherbrooke, 

Quebec J1E 4K8, Canada.


3
Department
of Plant Pathology, College of Agriculture, University
of Agricultural Sciences, Raichur – 584104, Karnataka,
India.


4
NRC
on DNA finger printing, NBPGR, PUSA Campus, New Delhi –
110012 India.

 


1
Email:
chinnagurikar @ gmail. com

 

*Corresponding Author’s Email:
sreenivasamy @ gmail. com,
Fax: +91-821-2411208;

Telephone: +91-821-2419733

Abstract:


Azotobacter

strains were isolated from paddy soils by serial dilution
agar plate method.  The colonies were glistening; smooth,
slimy, brown to black in morphology on Jenson’s N-free agar
plates and the cells were Gram
negative bacteria. Biochemically, they were positive for
indole production, citrate, catalase and Voges Proskauer
test. Further, Nif gene sequence results revealed the
presence of seven Azotobacter species. The effect of
1, 3 and 5% pesticides concentration viz., pendimethalin,
chloropyrifos, glyphosate and phorate on nitrogen fixation,
indole acetic acid, gibberllic acid, phosphate
solubilization and bioassay of Azotobacter sp. were
studied. Among all the species, GVT-1 strain was found to
fix nitrogen at a maximum of 30µgN2 ml-1
day-1, produced highest quantity gibberllic acid
(10µg25ml-1) and able
to
solubilize the phosphate at the rate of
9.8cm by forming the halo zone which was supplemented with
5% phorate. Similarly, GVT-1 strain produced a highest
amount of indole acetic acid (31.8µgml-1) in 5%
pendimethalin. Further bioassay activities of GVT-1 strain
found efficient in increasing the root, shoot length and
vigor of the plant. From these results it is clear that the
Azotobacter sp. not only produces plant growth
promoting substances but

is
also resistant
to different pesticides and
is

not affected
by
the bacterial activity.

Keywords: Azotobacter; Nif gene; PGP
activity; Bioassay; Pesticides.

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