Gurmessa And Asefa


Gurmessa and Asefa

Journal of Agricultural Sciences

Vol. 9(3), pp. 322-336, 2019

ISSN: 2276-7770

Copyright ©2019, the copyright of this article is
retained by the author(s)

DOI Link:



Nutrient Compositions and
Optimization of Elephant Grass (Pennisetum purpureum) Stem to Cotton Seed Proportion for the Cultivation of Oyster
Mushroom (Pleurotus ostreatus) at
Ambo Western, Ethiopia.


Gurmessa Tesema*; Asefa Keneni


Department of Biology, College of Natural and Computational Sciences, 

Ambo University, Ethiopia.






Article No.:070119123

Type: Research

DOI: 10.15580/GJAS.2019.3.070119123



present more emphasis has been given to mushroom cultivation for the
nutrition and medicinal uses and Agricultural product recycling technology.
The aim of this study was to investigate the usability of Pennisetum
purpureum stem as major substrate for cultivation of oyster (Pleurotus
ostreatus) mushroom with supplement of different proportion of cotton seed
waste. The culture of the oyster mushroom was maintained on potato dextrose
agar, and the spawn was prepared on yellow collared sorghum and sterilized
substrate was inoculated with 10% of the spawn wet basis on dry basis of the
substrate. The experimental design constitutes ten treatments on the stem of
elephant grass with ratios of cotton seed wastes (T1-T10) in three
replications. At T4 (70:30) the fastest mycelial grow on the stem of
elephant grass and Slowest mycelia extension were observed on T7
(60:40).  the highest fresh weight
were observed on T4 (70:30) stem of elephant were recorded 1254 g / 500g dry
substrate, highest number of fruits recorded on T5 (62) while largest cap
diameter (11.5cm) was recorded on T2 of the stem of elephant grass the
highest stipe length were recorded on T9 (3.67cm) . The highest biological
efficiency observed on T4 (250.5%) on stem of elephant grass. the highest
protein contents were recorded on T7 (36.17%) on stem of elephant grass with
supplementation of cottonseed wastes and the least one were   recorded on T9 (80:20) 16.87%, on the
others treatments intermediate number of food contents were recorded. Even
though on the stem of elephant grass and the proportion supplementation of
cotton seed waste was varied for all the treatments except on T1 treatments
make 100% Pennisetum purpureum dry stem were made. Over all, the results of
the study showed that the Pennisetum purpureum stem supplemented with cotton
seed wastes can be gave highest yield and yield related parameters of oyster
mushroom. The results of the present study implies to carry out further
research on the optimization of Pleurotus ostreatus production using two or
more type of substrates tested in this experiment.


Submitted: 01/07/2019

Accepted:  09/07/2019

Published: 23/09/2019


*Corresponding Author

Gurmessa Tesema

E-mail: gurmesat2014@


Keywords: biological
efficiency; Cottonseed wastes; Pennisetum purpureum; Pleurotus ostreatus.




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Cite this Article: Gurmessa, T; Asefa, K
(2019). Nutrient Compositions and Optimization of Elephant Grass (Pennisetum purpureum) Stem to Cotton
Seed Proportion for the Cultivation of Oyster Mushroom (Pleurotus ostreatus) at Ambo Western, Ethiopia. Greener Journal of Agricultural Sciences
9(3): 322-336,


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