Gurmessa And Asefa

Gurmessa and Asefa

Greener
Journal of Agricultural Sciences

Vol. 9(3), pp. 322-336, 2019

ISSN: 2276-7770

Copyright ©2019, the copyright of this article is
retained by the author(s)

DOI Link: https://doi.org/10.15580/GJAS.2019.3.070119123

http://gjournals.org/GJAS

  

 

Nutrient Compositions and
Optimization of Elephant Grass (Pennisetum purpureum) Stem to Cotton Seed Proportion for the Cultivation of Oyster
Mushroom (Pleurotus ostreatus) at
Ambo Western, Ethiopia.

  

Gurmessa Tesema*; Asefa Keneni

  

Department of Biology, College of Natural and Computational Sciences, 

Ambo University, Ethiopia.

 

 

ARTICLE INFO

ABSTRACT

 

Article No.:070119123

Type: Research

DOI: 10.15580/GJAS.2019.3.070119123

 

 

At
present more emphasis has been given to mushroom cultivation for the
nutrition and medicinal uses and Agricultural product recycling technology.
The aim of this study was to investigate the usability of Pennisetum
purpureum stem as major substrate for cultivation of oyster (Pleurotus
ostreatus) mushroom with supplement of different proportion of cotton seed
waste. The culture of the oyster mushroom was maintained on potato dextrose
agar, and the spawn was prepared on yellow collared sorghum and sterilized
substrate was inoculated with 10% of the spawn wet basis on dry basis of the
substrate. The experimental design constitutes ten treatments on the stem of
elephant grass with ratios of cotton seed wastes (T1-T10) in three
replications. At T4 (70:30) the fastest mycelial grow on the stem of
elephant grass and Slowest mycelia extension were observed on T7
(60:40).  the highest fresh weight
were observed on T4 (70:30) stem of elephant were recorded 1254 g / 500g dry
substrate, highest number of fruits recorded on T5 (62) while largest cap
diameter (11.5cm) was recorded on T2 of the stem of elephant grass the
highest stipe length were recorded on T9 (3.67cm) . The highest biological
efficiency observed on T4 (250.5%) on stem of elephant grass. the highest
protein contents were recorded on T7 (36.17%) on stem of elephant grass with
supplementation of cottonseed wastes and the least one were   recorded on T9 (80:20) 16.87%, on the
others treatments intermediate number of food contents were recorded. Even
though on the stem of elephant grass and the proportion supplementation of
cotton seed waste was varied for all the treatments except on T1 treatments
make 100% Pennisetum purpureum dry stem were made. Over all, the results of
the study showed that the Pennisetum purpureum stem supplemented with cotton
seed wastes can be gave highest yield and yield related parameters of oyster
mushroom. The results of the present study implies to carry out further
research on the optimization of Pleurotus ostreatus production using two or
more type of substrates tested in this experiment.

 

Submitted: 01/07/2019

Accepted:  09/07/2019

Published: 23/09/2019

 

*Corresponding Author

Gurmessa Tesema

E-mail: gurmesat2014@
gmail.com

 

Keywords: biological
efficiency; Cottonseed wastes; Pennisetum purpureum; Pleurotus ostreatus.

 

 

 

Return to Content       View [Full Article – PDF]  

[Full Article – HTML]              [Full Article – EPUB]

Post-Publication Peer-review Rundown

View/get involved, click [Peer-review]

 


REFERENCES

 

Alam, N.,
Khan, A., Hossain, M.S., Amin, S.M.R. and Khan, L.A. (2007). Nutritional
analysis of dietary mushroom Pleurotus
Florida Eger
and Pleurotus sajorcaju
Fr. Singer. Bangladesh J. Mushroom. 1
(2), 1-7.

 

Asefa, K
and Geda, K. (2014b). Cultivation of Oyster mushroom (Pleurotus ostreatus) on Waste Paper with Supplements’ of Wheat
Bran. Global J Res. Med. Plants and Indigen. Med. 3: 370–380

 

Ashraf, J,
Asif, AM, Ahmad, W, Muhammad, A C, and Shafi, J. (2013).  Effect of Different Substrate Supplements on
Oyster Mushroom (Pleurotus spp.)
Production Food Science and Technology 1(3): 44–51,

 

Atikpo,
M, Onokpise, O, Abazinge, M, Louime, C, Dzomeku, M, Boateng, L Awumbilla.
(2008). Sustainable mushroom production in Africa: A case study in Ghana. Afr. J. Biotechnol. 7:249 –253.

 

Beetz,
A, Kustudia, M. (2004). Mushroom cultivation and marketing: Horticulture
production guide. Horticulture Production Guide, ATTRA Publication IP087.

 

Bernardi,
E, Donini L.P, Minotto E, Nascimento J.S. (2009). Cultivo characteristics
nutricionais de Pleurotus substrato
pasteurized. Bragantia 68.901-907.

 

Bhuyan.
(2008). Study on preparation of low cost spawn packets for the production of
oyster mushroom (Pleurotus Ostreatus)
and its proximate analysis. M.S. Thesis, Department of Biochemistry, SAU,
Dhaka, Bangladesh.

 

Breene,
W. (1990).Nutritional and medicinal value of mushrooms. Journal of Food Production, 53:883-894.

 

Chang
S-T. (1999). World Production of Cultivated Edible and Medicinal Mushrooms in
1997 with Emphasis on Lentinus edodes (Berk.) Sing, in China. International Journal of Medicinal Mushrooms.1
(4):291-300.

 

Chang,
S.T. and Bus well, J.A. (2003). Mushrooms-A prominent source of nutraceuticals
for the 21st Century. Curr. Topics in Nutraceuticals Res.1, 257-280.

 

Chang,
S.T., Lau, O.W. and Cho, K.Y. (1981).The cultivation and nutritional value of Pleurotus sajor-caju.Eur. J. Appl. Microbiol. Biotechnol. 12
(1): 58-62.

 

Chang, S-T.(1999).
World Production of Cultivated Edible and Medicinal Mushrooms in 1997 with
Emphasis on Lentinus edodes (Berk.)
Sing, in China. International Journal of
Medicinal Mushrooms.
1(4):291-300.

 

Cook,B.G,pengelly,B.C,Brown,S.D,Donnely,J.L,Eagles,D.A,Franco,M.A,Hanson,J,Mullen,B.F,partridge,I.J,peters,M,schultle-kraft,R.(2005).
Tropical forages, CSDIRO, DPI and F (QID), CIAT and ILRI, Brisbane, Australia.

 

Crisan, EW and Sands. (1978). A
Nutritional value.  In:  Chang ST and Hayes WA (Eds.).  The biology and cultivation of edible
mushrooms. Academic press, New York.172-189.

 

Dawit,
A. (1998). Mushroom cultivation. A practical approach. Berhanena Selam printing
press. Addis Ababa. Ethiopia.

 

Deepalakshmi and Mirunalini, S.
(2014). “Pleurotus ostreatus: An
oyster mushroom with nutritional and medicinal properties,” Journal of Biochemical Technology, vol.
5:718-726.

 

Diriba, M, Beje, G and
Dawit, A. (2013).Evaluation of locally available substrates for cultivation of
oyster mushroom (Pleurotus ostreatus).African journal of Microbiology Research
Jimma, Ethiopia
. 2228-2237.

 

FAO.(2015).Grass land index. Searchable catalogue of grass and
foragelegumsas, FAO, Rome, Italy

 

FAO
(Food and Agriculture Organization). (2009).Making money by growing mushrooms,
Diversification booklet number 7.Accessed on 06/06/2014 from
ftp://ftp.fao.org/docrep/fao/011/i0522e/i0522e00.pdf.

 

Folch, J., Lees, M.
and Sloane-Stanely, and G.H. (1957).A simple method for the isolation and
purification of total lipids from animal tissues. J. Biol. Chem., 226: 497–509.

 

Godfrey,
E.Z, Siti, M.K, Judith, Z.P. (2010). 
Effects of temperature and hydrogen peroxide on mycelial growth of eight
Pleurotus strains. Scientia
Horticulture, v.125, p.95
102, 2010.
DOI: 10.1016/j.scienta. 03.006.

 

Gume, B, Muleta, D and Dawit, A.
(2013).Evaluation of locally available substrates for cultivation of oyster
mushroom (Pleurotus ostreatus) in
Jimma, Ethiopia. African Journal of
Microbiology Research
7(20):2228-2237

 

Hassan, F and Medany, G. M. (2014). Effect of pretreatments and drying temperatures on the quality
of dried Pleurotus mushroom SPP
Egypt. Journal of
Agriculture Research
92: 1009–1023

 

Islam,
M.Z, Rahman, M.H, and Hafiz, F. (2009).Cultivation of oyster mushroom (Pleurotus flabellatus) on different
substrates .Int. J. Sustain. Crop Prod.
4:45-48

 

Kalac,
P. (2009) Chemical composition and nutritional values of European species of
wild growing mushrooms: A review. Food Chem 113: 9-16

 

Khan, M.A.
(2010) Nutritional composition and Hypocholesterolemic effect of mushroom: Pleurotus sajor-caju and Pleurotus Florida: LAP Lambert Academic
publishing Gmbh &co. KG: Saarbrucken, Germany 1-11

 

Kimenju,
J.W, Odero, O.M, Mutitu, E.W, Wachra, P.M, Narla, R.D, and Muiru, W.M.
(2009).Suitability of locally available substrates for oyster mushroom (Pleurotus ostreatus) cultivation in
Kenya. Asian J. Plant Sci.,
8:510–514.

 

Mandeel
Q, Al-Laith A, Mohamed S. (2005). Cultivation of oyster mushrooms (Pleurotus
spp.) on various lignocellulosic wastes. World
Journal of Microbiology and Biotechnology.
21 (4):601-7.

 

Masarirambi,
M. Mamba, and D. Earnshaw.(2011). “Effect of various substrates on growth
and yield of oyster mushrooms,” Asian J. Agric. Sci., vol. 3, pp.
375-380, 2011.

 

Mdconline. (2013). Oyster mushroom, Missouri Department of
Conservation, Accessed on 02/04/2013 from http://mdc.mo.gov/node/20763

 

Mekonnen,
H and Semira.(2014). Suitability of locally available substrates for oyster
mushrooms cultivation in Mekelle City, Tigray, Ethiopia. Sky Journal of Food Science 3(5):047 – 051

 

Moni, K.H.,
Ramabardan, R. and Eswaran, A. (2004). Studies on some physiological, cultural
and post harvest aspects of oyster mushroom Pleurotus
ostreatus
(berk). Trop: Agril. Res. 12: 360-374

 

Morais, M. H., Ramos,
A. C., Matos, N., and Oliveira, E. J. S. (2000). Note: Production of shiitake
mushroom (Lentinus edodes) on lignin cellulosic residues. Food
Science and TechnologyInternational
.6:123-128.

 

Mkhize,
S. S., Cloete, J., Basson, A. K., and Zharare, G. E. (2016). Performance of Pleurotus
ostreatus
mushroom grown on maize stalk residues supplemented with various
levels of maize flour and wheat bran. Food Science and Technology,
36(4), 598-605.

 

Nunez, J.
P., and Mendoza, C. G. (2002). Submerged fermentation of Ligno-cellulosic
wastes under moderate temperature conditions for oyster mushroom growing
substrates. Mushroom Biology and Mushroom Products, 5:545−549.

 

Oei
P, van Nieuwenhuijzen, B. (2005). Small-scale mushroom cultivation: oyster,
shiitake and wood ear mushrooms. Wageningen, the Netherlands.

 

Ogundele, G. F., Salawu, S. W
Abdulraheem, I. A., and Bamidele, O. P. (2017).Nutritional Composition of
Oyster Mushroom (Pleurotus ostreatus)
Grown on Softwood   (Daniella oliveri) Sawdust and Hardwood (Anogeissus leiocarpus) Sawdust. British
Journal of Applied Science and Technology,
20(1): 1-7.

 

Olfati,
J.A, Peyvast, G.H. (2012). Lawn clippings for cultivation of Oyster Mushroom. Int. J. Vegetable Sci., 14: 98-103.

 

Olumide, O.J. (2007).
Economic analysis of mushroom marketing as a copping strategy for poverty
reduction in Ondo State, Nigeria. Afr. Crop Sci. Conf. Proc. 1255-1260.

 

Oseni,T.O, Dube,S.S, Wahome,p.K,masarirabi,M.T,and
Earnshaw,D.M.(2012). Effect of wheat Bransupplement on growth and Yield of  Oyster mushroom (Pleurotus Ostreatus) onFermented pine saw dust substrate.Experimental Agriculture:V-30-40.

 

Oyetayo, V.O and Ariyo, O.O.
(2013).Micro  and  macronutrient 
properties  of  Pleurotus  ostreatus 
(Jacq:  Fries)  cultivated 
on  different  wood substrates.  Jordan
J.  Biol. 
Sci.
6:223-226

 

Patra,
A., and Pani, B. (1995).Evaluation of banana leaf as a new alternative
substrate to paddy straw for oyster mushroom cultivation. Journal of Phytological Research,
8, 145-148.

 

Philippoussis,
A.G, Diamantopoulos, P. (2012). Bioconversion of Agricultural lignocellulosic
wastes through the cultivation of edible mushrooms Agrocybeaegerita,
Volvariella volvacea and Pleurotus spp.
World J. Microbiol. Biotechnol. 17:
191-200.

 

Raghuramalu, N.,
Madhavan, N. K. and Kalyanasundaram, S. (2003).A Manual of Laboratory
Techniques. National Institute of Nutrition. Indian Council of Medical
Research, Hyderabad-500 007, India.56-58.

 

Reguła, J and Siwulski, M. (2007). Dried shiitake (Lentinula
edodes
) and oyster (Pleurotus ostreatus) mushrooms as a good source of nutrientActa Scientiarum Polonorum Technologia Alimentaria6:135–142.

 

Sara,
M. (2007).Effect of spawn substrates and their inoculation rates on the yield
and quality of oyster mushroom (Pleurotus
Florida
). M.Sc. Thesis Presented to the School of Graduate Studies of
Haramaya University.  22-23.

 

Sarker, N.C.
(2004). Oyster mushroom (Pleurotus
ostreatus
) Production Technology Suitable for Bangladesh and its Nutritional
and Postharvest Behavior. PhD Thesis, Bangabandhu Sheikh Mujibur Rahman
Agricultural University, Gazipur, Bangladesh

 

Shah, Z.
A., Ashraf, M., and Ishtiaq, Ch. (2004). Comparative study on cultivation and
yield performance of Oyster mushroom (Pleurotus ostreatus) on different
substrates wheat straw, leaves, saw dust. Pakistan
J. Nutr
., 3, 158–160.

 

Silva,
E.G, Dias, E.S, Siqueira, F.G, and Schwan, R.F. (2007).Análise química de
corpos de frutificação de Pleurotus sajor-caju cultivado diferentes concentrações
de nitrogen. Ciência e Tecnologia de Alimentos27: 72-75.

 

Stamets,
P. (2009). Growing Gourmet and Medicinal Mushrooms 3rd Edn. Ten Speed Press,
Berkeley, California, pp: 574.

 

Teklit, G. A. (2015) Chemical Composition and Nutritional
Value of the Most Widely Used Mushrooms Cultivated in Mekelle Tigray Ethiopia. J Nutr Food Sci 5:408.

 

Tuno,
N. (2001). Mushroom utilization by the Majangir, an Ethiopian tribe. Laboratory
of insect ecology: Graduate school of agriculture, Kyoto University, Japan.

 

Vidya,
J.T., Sobita Simon and Abhilasha ,A. (2017).Efficacy of different substrates on
the growth, yield and nutritional composition of oyster mushroom- Pleurotus Florida (Mont.) Singer. Journal of Pharmacognosy and Photochemistry, 6(4):
1097-1100.

 

Wani,
B.A, Bodha, R.H, Wani, A.H. (2012). Nutritional and medicinal importance of
mushrooms. J. Med. Plants Res., 4
(24): 2598-2604.


Cite this Article: Gurmessa, T; Asefa, K
(2019). Nutrient Compositions and Optimization of Elephant Grass (Pennisetum purpureum) Stem to Cotton
Seed Proportion for the Cultivation of Oyster Mushroom (Pleurotus ostreatus) at Ambo Western, Ethiopia. Greener Journal of Agricultural Sciences
9(3): 322-336, https://doi.org/10.15580/GJAS.2019.3.070119123

 

Leave a Reply

Your email address will not be published. Required fields are marked *