Konate Et Al

Konate et al

Greener Journal of Agricultural Sciences Vol. 5 (1), pp. 043-051, February 2015.

 ISSN: 2276-7770  

Research Paper

Manuscript Number: 121114393

DOI: http://doi.org/10.15580/GJAS.2015.1.121114393

Screening of Endophytic Bacteria Associated with Ceratonia siliqua L. Plant Using
Molecular Marker Repetitive Extragenic Palindromic (Rep)-PCR

 

Ibrahim Konate1,*,
Mathurin Koffi1, Annick Koulibaly1, Amina Sorouri2,
El Bekkay Berraho2, Abdelkarim Filali-Maltouf2

 

1Unité de Formation
et de Recherche en Agroforesterie et en Environnement, Laboratoire des
Interactions Hôte-Microorganisme, Environnement et Evolution (LIHME),
Université Jean Lorougnon Guédé, B.P: 150 Daloa, Côte d’Ivoire.

2Faculty
of Sciences, Laboratory of Microbiology and Molecular Biology, University
Mohammed V-Agdal, Rabat, Morocco.

 

*Corresponding
author’s email: konatibrahim@ yahoo.fr
, BP: 150
Daloa (Côte d’Ivoire),

Fax: (+225) 32 78 75 70

Abstract

 

Repetitive extragenic palindromic
(Rep)-PCR was used to screen and to characterize the natives endophytic
bacteria isolated from roots (IRC) and epicotyls (IEC) of young carob (Ceratonia siliqua L.) plant collected
from different Moroccan localities. Genomic DNA of 80 bacteria (69 IRC and 11
IEC), 7 strains of RCM and 13 strains of reference rhizobia were successfully
carried. The complementary primers of Rep induced reproducible PCR fingerprint
patterns and generated more bands polymorphs and useful for distinguishing the
isolates from each other. We obtained respectively 6, 17 and 35 polymorphic
bands with IEC, RCM and IRC strains with the size ranging between 380 to 7250
pb. Dendrogram based upon UPGMA analysis of Rep-PCR patterns showed high degree
of genetic diversity among indigenous carob endophytic bacteria. At 87 % of
similarity, we revealed a wide polymorphism and obtained 2 greats clusters, 6
smalls groups and 24 independent lines. Rep-PCR technology allowed us, firstly
to characterize 14 IRC genetically belonging to RMC, Sinorhizobium sp, Rhizobium
sp and Agrobacterium sp; and
secondly, to revalue the exact number of our collection to 45 strains and to
select excessively34 endophytic bacteria original from seven Moroccan regions
for futures works.     

 

Key words: Enophytic bacteria, Carob (Ceratonia siliqua L), REP-PCR, screen,
genetic diversity.


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Cite this Article: Konate I,
Koffi M, Koulibaly A, Sorouri A, Berraho EB, Filali-Maltouf A, 2015. Screening
of Endophytic Bacteria Associated with Ceratonia siliqua L. Plant Using
Molecular Marker Repetitive Extragenic Palindromic (Rep)-PCR. Greener Journal
of Agricultural Sciences, 5(1):043-051,
http://doi.org/10.15580/GJAS.2015.1.121114393.

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