Madakbas Et Al

Madakbaş et al



Greener
Journal of  Agricultural Sciences


Vol. 4 (2), pp.
039-045,
March 2014.


 ISSN: 2276-7770 



Research

Paper

Manuscript Number: 021014101

DOI: http://dx.doi.org/10.15580/GJAS.2014.2.021014101

 



Determination of Phaseolin
Types
 in
Common Bean (Phaseolus vulgaris)
Varieties from Turkey

                                                                                                     



Seher Yıldız Madakbaş1*, Halima Awale2
and



James Kelly2

 


1
Ahi
Evran University, Agricultural Faculty, Department of
Horticulture, Kırşehir-Turkey.

2Michigan State University,
Department of Crop, Soil and Microbial Sciences,
Michigan- USA.

 

*Corresponding
Author’s Email: seheryldzmadakbas @ gmail.com

Abstract:

The purpose of
our study was to determine the phaseolin types of common
bean varieties widely consumed in Turkey through protein
extraction. Twenty- four bean genotypes, consisting of 22
varieties from Kırsehir province of Turkey and two control
varieties (Andean and Mesoamerican) were assessed. Sodium
dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE)
was performed with protein samples extracted from bean
genotypes. Gels were stained with Coomassie blue dye R-250
and kept in fixing solution. Our analyses revealed that out
of 22 bean samples, 16 contained Andean phaseolin type and 6
contained Mesoamerican phaseolin type. T-test statistical
analysis was performed to determine the relationship between
seed phaseolin types and seed volumes (length, height and
width). The results showed that there was a significant
difference between phaseolin and seed volume.
 
Keywords: Phaseolus vulgaris, phaseolin, SDS-PAGE,
genotype, Andean, Mesoamerican, seed volume.

Reference:


Acquaah G, Isleib TG (1994). Gene pool specificity,
paucity of enzyme variation and phaseolin polymorphism in the common bean.
HortScience. 29:1337–1339.

Ahn J, Sen
CL, Whitaker JR (1991).  Stability of tertiary structure of  Phaseolin of 
Red  Kidney  Bean (Phaseolus vulgaris) as  limiting factor  in proteolysis. Journal of Food
Biochemistry. 15: 263-278.

Angioi SA,
Rau D, Attene ,  Nanni L, Bellucci E,
Logozzo G, Negri V, Spagnoletti Zeuli PL, Papa R, (2010). Beans in  Europe: origin and structure of the European
landraces of Phaseolus vulgaris L.
Theor Appl  Genet. 121:829–843.

Bollini R,
Chrispeels MJ (1978). Characterization and subcellular localization of vicilin
and phytohemagglutinin, the two major reserve proteins of Phaseolus vulgaris
L. Planta. 142: 291-298.

Bradford MM
(1976). A rapid and sensitive method for the quantitation of microgram
quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry. 72: 248-254.

Brown  JWS, Bliss FA 
Hall TC (1981a). Linkage relationships between genes controlling seed
proteins in French bean. Theor Appl
Genet 60: 251-259.

Brown JWS,
Ma Y, Bliss FA  Hall TC (1981b).  Genetic variation in the subunits of
globulin-1 storage protein of French bean. Theor Appl Genet 59: 83–88.

Chacon  MI, Pickersgill  SB, Debouck 
DG (2005). Domestication patterns in common bean (Phaseolus vulgaris L.) and the origin of the Mesoamerican and
Andean cultivated races. Theor Appl Genet. 110: 432–444.

Chandrakanth
E, Hall TC (2008). Phaseolin: structure
and evolution. The Open
Evolution Journal. 2: 66-74.

De la
Fuente M, María López-Pedrous M, Alonso J, Santalla M, de Ron A.., Álvarez G,
Zapata C (2012).
In-depth characterization of the Phaseolin protein diversity  of Common Bean (Phaseolus vulgaris L.)
based on two-dimensional electrophoresis and mass spectrometry.
Food
Technol Biotechnol.  50
(3): 315–325.

Derbyshire  E,  Wright  D J,  Boulter  D (1976).
Legumin and Vicilin, storage proteins of 
legume seeds. Phytochemistry.
15: 3-24.

FAO  (2011).
www.fao.stat.fao.org

FAO  (2012).
www.fao.stat.fao.org

Gepts
P,  Bliss F A (1986).  Phaseolin variability among wild and
cultivated Common Beans (Phaseolus vulgaris) from Colombia. Economic Botany. 40(4):  469-478.

Gepts P,
Osborn TC,   Rashka K, Bliss FA
(1986).  Phaseolin-protein variability in
wild forms and landraces of the common bean (Phaseolus vulgaris):
evidence for multiple centers of 
domestication. Econ  Bot. 40: 451–468.

Gepts P, Bliss
FA, (1988). Dissemination pathways of
Common Bean (Phaseolus vulgaris, Fabaceae) deduced from Phaseolin
electrophoretic variability. II. Europe and Africa. Economic 
Botany.  42(1): 86-104.

Gepts  P (1993). The use of molecular and
biochemical markers in crop evolution studies. Evol Biol. 27: 51-94.

Gepts P
(1998). Phaseolin as an evolutionary marker. In: genetic resources of Phaseolus beans, P. Gepts (Ed.),
Kluwer Academic Publishers, Dordrecht, The Netherlands, 215–241.

Kaplan  L (1965). 
Archeology and domestication in American Phaseolus (beans). Econ
Bot. 19: 358-368.

Kawi J,
Velasquez VB, Debouck GD, Gepts P (1995). Identification of presumed ancestral
DNA sequences of phaseolin in Phaseolus
vulgaris
. Proc  Natl  Acad Sci. 92, 1101-1104.

Koenig  RL, Singh SP 
Gepts P (1990). Novel phaseolin types in wild and cultivated common bean
(Phaseolus vulgaris, Fabaceae).Econ  Bot. 44: 50–60.

Laemmli VK
(1970). Cleavage of structure proteins during assembly of the head of
bacteriophage T4. Nature.  22: 680-685.

Lareo LR, Gonzalez AV, Barona E,
Beebe S (1993).
Fast and reliable one and two–dimensional
electrophoretic parameters for phaseolin type identification. Annu Rept Bean
Improvement Coop (USA). 36: 8-9.

Ligarreto G, Ocampo C (2012). Genetic diversity in a Colombian bean (Phaseolus
vulgaris
L.) collection as assessed by phaseolin patterns and isoenzymatic
markers
. Agronomía Colombiana. 30(2): 179-187.

Limongelli
G, Laghetti G, Perrino P, Piergiovanni AR (1996). Variation of seed storage
proteins in landraces of common bean (Phaseolus
vulgaris
L.) from Basilicata, Southern Italy. Euphytica. 92: 393–399.

Lioi  L(1989). Variation of the storage protein
phaseolin in common bean (Phaseolus
vulgaris
L.) from the Mediterranean area. Euphytica.  44 : 151 -155.

Logozzo G,
Donnoli R, Macaluso L, Papa R, Knupffer H, Spagnoletti Zeuli  PL (2007). Analysis of the contribution of
Mesoamerican and Andean gene pools to European common bean (Phaseolus vulgaris L.) germplasm and
strategies to establish a core collection. Genet Resources Crop Evol.
54:1763–1779.

Ma Y, Bliss F (1978).  Seed proteins of common bean. Crop Sci.  18: 431–437.

Maras M,
Sustar-Vozlic J (2013). Genetic diversity and dissemination pathways of Common
Bean in Central Europe. J   Amer   Soc  
Hort  Sci.  138(4):297–305.

Mcleester RC,  Hall T C, Sun SM, Bliss  F- A (1973). Comparison of globulin proteins from Phaseolus
vulgaris
with those from Vicia  faba. Phytochemistry. 2:85- 93.

Perez-Vega
E, Campa A,  De la Rosa L, Giraldez  R, Ferreira JJ (2009). Genetic diversity in a
core collection established from the main bean genebank in Spain. Crop Sci.
49:1377–1386.

Piergiovanni
AR, Taranto G. and  Pignone D. (2000).
Diversity among common bean populations from the Abruzzo region (Central
Italy): a preliminary inquiry. Genet Resources Crop Evol 47:467–470.

Rodiňo
P, Monteagudo AB, Santalla M  (2001).
Methodology for electrophoretic analysis of Phaseolin.
Ed: Jacobsen HJ, Muzquiz M, Hassa A, Handbook on Common Bean Related Laboratory
Methods. PHASELIEU – FAIR – PL97-3463, 
Misión Biológica de Galicia (CSIC).

Rodiňo 
AP, Santalla M, Montero I, Casquero PA 
de Ron AM (2001). Diversity in common bean (Phaseolus vulgaris L.)
germplasm from Portugal. Genet Resour 
Crop Evol.  48: 409–417.

Rodiňo P, Santalla M, de Ron AM, Singh  SP ( 2003). A core collection of common bean
from the Iberian Peninsula. Euphytica. 131:165–175.

Rodiňo AP, Gonzalez AM, Santalla  M, De Ron 
AM Singh SP (2006). Novel genetic variation of the common bean in
Europe. Crop Sci. 46:2540–2546.

Rodiňo A P, Monteagudo AB, de Ron A M
Santalla M (2009).
Ancestral landraces
of common bean from the South of Europe and their agronomical value for
breeding programs.
Crop Science. 49:2087-2099.

Romero J,
Sun SM, McLeester RC, Bliss FA, Hall TC (1975). Heritable variation in a
polypeptide subunit of the major storage protein of the bean (Phaseolus
vulgaris
L.). Plant Physiol. 
56:776-779.

Santalla M, Rodino AP, de Ron AM (2002). Allozyme
evidence supporting southwestern Europe as a secondary center of genetic
diversity for the common bean. Theor Appl Genet. 104: 934–944.

Singh SP, Gutierrez JA, Molina A, Urrea C, Gepts  P (1991). Genetic diversity in cultivated
common bean. II. Marker-based analysis of morphological and agronomic traits.
Crop Sci.  31:23–29.

Singh  SP (2001). Broadening the genetic base of
common bean cultivars: A review.  Crop Sci. 
41:1659–1675.

Stonehouse J M, Forrester GJ (1998). Robustness of the t
and U  tests under combined
assumption  violations. Journal of
Applied   Statistics. 25: 63–74.

Sustar-Vozlic
J, Maras M,, Javornik B,  Meglic V
(2006). Genetic diversity and origin of Slovene common bean ( Phaseolus vulgaris L.) germplasm as
revealed by AFLP markers and phaseolin analysis. J  Amer 
Soc  Hort  Sci. 131:242–249.

Toro O, Ocampo  
CH., Debouck DG (2007).
Phaseolin: variability and reference materials in wild  and cultivated common bean. Annu  Rept Bean Improvement Coop (USA).  50: 69-70.  

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