Mensah et al Greener Journal of Agricultural Sciences Vol. 10(2), pp. 57-62, 2020 ISSN: 2276-7770; Copyright ©2020, the copyright of this article is retained by the author(s) http://gjournals.org/GJAS Effects of GA3, BAP and KNO3 on the Germination and DNA Content of Cucumber (Cucumis sativus L.) Mensah, S.I.; Ejeagba, P.O.; Okonwu, K. Department of Plant Science and Biotechnology, University of Port Harcourt, P.M.B. 5323, Port Harcourt, Nigeria. ARTICLE INFO ABSTRACT Article No.: 021720037 Type: Research Effects of gibberellic acid (GA3), 6-benzylaminopurine (BAP) and potassium nitrate (KNO3) on the seed germination and DNA concentration of cucumber (Cucumis sativus) radicle were assessed. The concentrations of these growth stimulants were 0 mM, 1 mM, 5 mM and 10 mM. The cucumber seeds were surface sterilized in ethanol for 5 minutes and rinsed with distilled water before pretreatment with these growth stimulants. The germination study was allowed to stand for 14 days and DNA concentration of cucumber radicle with the highest germination count was determined for each growth stimulant. The study showed that cucumber seeds had higher germination count under the light condition than in the dark condition. However, it is not statistically different. The study also showed that percentage germination of cucumber seeds was enhanced by GA3 (57 – 72%) and BAP (62 – 70%) when compared to the Control (50%) except KNO3 (41 – 44%). Across the treatments, GA3 gave the highest germination percentage followed by BAP with 5 mM concentration producing the highest germination count while 10 mM recorded the highest in KNO3. The DNA concentration of the cucumber radicle that produced these highest germination percentage are: GA3 (47.40 ng/µl), BAP (98.87 ng/µl), KNO3 (103.23 ng/µl) and Control (79.73 ng/µl). The analysis of variance (ANOVA) showed that treatments are significant at p-value (0.0001) < 5% significant level for cucumber seed. The study recommends the use of 5 mM GA3 in germinating cucumber seeds. Accepted: 19/02/2020 Published: 25/04/2020 *Corresponding Author Okonwu, K. E-mail: kalu.okonwu@ uniport.edu.ng Keywords: growth stimulants; concentration; germination; cucumber seed Return to Content View [Full Article – PDF] [Full Article – HTML] [Full Article – EPUB] Post-Publication Peer-review Rundown View/get involved, click [Peer-review] REFERENCES: Bewley JD, Hempel FD, McCormick S and Zambryski P (2000). In: Buchanan BB, Gruissem W, Jones RL, (eds). Biochemistry and Molecular Biology of Plants. American Society of Plant Physiologists, Rockville, MD; Pp. 988–1403. Bewley JD, Kent J, Bradford K, Hilhorst H and Nonogaki H (2013). Seeds: Physiology of Development, Germination and Dormancy, 3rd Edition. Springer New York; Pp. 1-399. Bino RJ, De-Vries JN, Kraak HL and Van-Pijlen JG (1992). Flow cytometric determination of nuclear DNA replication stages in tomato seeds during priming and germination. Annals of Botany; 69: 231-236. Chrispeels MJ and Varner JE (1967). Gibberellic acid-enhanced synthesis and release of α-amylase and ribonuclease by isolated barley aleurone layers. Plant Physiol., 42: 398-406. Finkelstein RR (2004). The role of hormones during seed development and germination. In: Davies, P.J. (Ed.), Plant Hormones: Biosynthesis, Signal transduction, Action! The Netherlands, Kluwer Academic Publishers, Dordrecht. Pp. 513–537. Jacobsen JV, Higgins TJV and Zwar JA (1979). Hormonal control of endosperm function during germination. In The Plant Seed. eds. I. Rubenstein, B. G. Gegenbach, R. L. Phillips and C. E. Green. Academic Press, New York, pp. 241-262. Jones RL (1973). Gibberellins: their physiological role. Ann. Rev. Plant Physiol. 24: 571-598. Jones RL and Armstrong JE (1971). Evidence for osmotic regulation of hydrolytic enzyme production in germinating barley seeds. Plant Physiol., 48: 137-142. Jones RL and Jacobsen JV (1982). The role of endoplasmic reticulum in the synthesis and transport of α-amylase in barley aleurone layers. Planta 156: 421-432. Mensah SI and Agbagwa IO (2001). The responses of seeds of Capsicum frutescens L. to the exogenous application of some growth promoters. J. Agric. Biotechnol. Environ., 3: 37-47. Mensah SI and Agbagwa IO (2004). Breaking dormancy in Gmelina arborea Roxb through treatment of seed with chemical substance and alternating temperature. Journal of Biological Research and Biotechnology; 2(1): 59 – 66. Miransari M and Smith DL (2009). Rhizobial lipochitooligossacharides and gibberellins enhance barley (Hordeum vulgare L.) seed germination. African Journal of Biotechnology, 8: 270-275. Miyoshi K and Sato T (1997). The effects of kinetin and gibberellin on the germination of dehusked seeds of Indica and Japonica rice (Oryza sativa L.) under anaerobic and aerobic conditions. Annals of Botany, 80: 479-483. Nonogaki H, Bassel G and Bewly J (2010). Germination still a mystery. Plant Science, 179: 574-581. Pinfield NJ and Stobart AK (1969). Gibberellic-stimulated nucleic and metabolism in the cotyledons and embryonic axes of Coryllus avellane (L.) seeds. New Phytol., 68: 993-999. Singh S, Singh P, Sanders DC and Wehner TC (2001). Germination of watermelon seeds at low temperature. Cucurbit Genetics Cooperative; 24: 59-64. Subedi KD and Ma BL (2005). Seed priming does not improve corn yield in a humid temperate environment. Agronomy Journal; 97: 211-218. Varner JE and Ho DT (1976). The role of hormones in the integration of seedling growth. In The Molecular Biology of Hormone Action. ed. J. Papaconstantinou. Academic Press, New York. pp. 173-194. Vishal B and Kumar PP (2018). Regulation of seed germination and abiotic stresses by gibberellins and abscisic acid. Front. Plant Sci., 9: 838 Yanyan LV, Qing MO, Alison AP and Yanrong W (2018). DNA replication during seed germination, deterioration and its relation to vigor in alfalfa and white clover. Crop Science; 58(3): 1393-1401. Zeb A, Khan A, Khan R and Shabbir F (2018). Effect of different concentrations of kinetin on seed germination in tomato. International Journal of Agronomy and Agricultural Research, 12(2): 1-8. Cite this Article: Mensah, SI; Ejeagba, PO; Okonwu, K (2020). Effects of GA3, BAP and KNO3 on the Germination and DNA Content of Cucumber (Cucumis sativus L.). Greener Journal of Agricultural Sciences 10(2): 57-62. .