Mensah Et Al


Mensah et al

Journal of Agricultural Sciences

Vol. 10(2), pp. 57-62, 2020

ISSN: 2276-7770;

Copyright ©2020, the
copyright of this article is retained by the author(s)








Effects of
GA3, BAP and KNO3 on the Germination and DNA Content of
Cucumber (Cucumis sativus L.)



S.I.; Ejeagba, P.O.; Okonwu, K.



Department of Plant
Science and Biotechnology, University of Port Harcourt,

P.M.B. 5323, Port Harcourt, Nigeria.






Article No.: 021720037

Type: Research



Effects of gibberellic
acid (GA3), 6-benzylaminopurine (BAP) and potassium nitrate (KNO3)
on the seed germination and DNA concentration of cucumber (Cucumis sativus)
radicle were assessed. The concentrations of these growth stimulants were 0
mM, 1 mM, 5 mM and 10 mM. The cucumber seeds were surface sterilized in
ethanol for 5 minutes and rinsed with distilled water before pretreatment
with these growth stimulants. The germination study was allowed to stand for
14 days and DNA concentration of cucumber radicle with the highest
germination count was determined for each growth stimulant. The study showed
that cucumber seeds had higher germination count under the light condition
than in the dark condition. However, it is not statistically different. The
study also showed that percentage germination of cucumber seeds was enhanced
by GA3 (57 – 72%) and BAP (62 – 70%) when compared to the Control
(50%) except KNO3 (41 – 44%). Across the treatments, GA3
gave the highest germination percentage followed by BAP with 5 mM
concentration producing the highest germination count while 10 mM recorded
the highest in KNO3. The DNA concentration of the cucumber radicle
that produced these highest germination percentage are: GA3 (47.40
ng/µl), BAP (98.87 ng/µl), KNO3 (103.23 ng/µl) and Control (79.73
ng/µl). The analysis of variance (ANOVA) showed that treatments are
significant at p-value (0.0001) < 5% significant level for cucumber seed.
The study recommends the use of 5 mM GA3 in germinating cucumber


Accepted:  19/02/2020

Published: 25/04/2020


*Corresponding Author

Okonwu, K.

E-mail: kalu.okonwu@



stimulants; concentration; germination; cucumber seed





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Cite this Article: Mensah, SI; Ejeagba, PO; Okonwu, K (2020).
Effects of GA3, BAP and KNO3 on the Germination and DNA Content of Cucumber (Cucumis sativus L.). Greener Journal of Agricultural Sciences
10(2): 57-62.



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