Mensah Et Al

Mensah et al

Greener
Journal of Agricultural Sciences

Vol. 10(2), pp. 57-62, 2020

ISSN: 2276-7770;

Copyright ©2020, the
copyright of this article is retained by the author(s)

http://gjournals.org/GJAS

 

 

 

 

 

 

 

Effects of
GA3, BAP and KNO3 on the Germination and DNA Content of
Cucumber (Cucumis sativus L.)

 

 

Mensah,
S.I.; Ejeagba, P.O.; Okonwu, K.

 

 

Department of Plant
Science and Biotechnology, University of Port Harcourt,

P.M.B. 5323, Port Harcourt, Nigeria.

  

 

ARTICLE INFO

ABSTRACT

 

Article No.: 021720037

Type: Research

 

 

Effects of gibberellic
acid (GA3), 6-benzylaminopurine (BAP) and potassium nitrate (KNO3)
on the seed germination and DNA concentration of cucumber (Cucumis sativus)
radicle were assessed. The concentrations of these growth stimulants were 0
mM, 1 mM, 5 mM and 10 mM. The cucumber seeds were surface sterilized in
ethanol for 5 minutes and rinsed with distilled water before pretreatment
with these growth stimulants. The germination study was allowed to stand for
14 days and DNA concentration of cucumber radicle with the highest
germination count was determined for each growth stimulant. The study showed
that cucumber seeds had higher germination count under the light condition
than in the dark condition. However, it is not statistically different. The
study also showed that percentage germination of cucumber seeds was enhanced
by GA3 (57 – 72%) and BAP (62 – 70%) when compared to the Control
(50%) except KNO3 (41 – 44%). Across the treatments, GA3
gave the highest germination percentage followed by BAP with 5 mM
concentration producing the highest germination count while 10 mM recorded
the highest in KNO3. The DNA concentration of the cucumber radicle
that produced these highest germination percentage are: GA3 (47.40
ng/µl), BAP (98.87 ng/µl), KNO3 (103.23 ng/µl) and Control (79.73
ng/µl). The analysis of variance (ANOVA) showed that treatments are
significant at p-value (0.0001) < 5% significant level for cucumber seed.
The study recommends the use of 5 mM GA3 in germinating cucumber
seeds.

 

Accepted:  19/02/2020

Published: 25/04/2020

 

*Corresponding Author

Okonwu, K.

E-mail: kalu.okonwu@
uniport.edu.ng

 

Keywords:

growth
stimulants; concentration; germination; cucumber seed

 

 

 

 

Return to Content       View [Full Article – PDF]  

[Full Article – HTML]              [Full Article – EPUB]

Post-Publication Peer-review Rundown

View/get involved, click [Peer-review]

 

REFERENCES:

 

Bewley
JD, Hempel FD, McCormick S and Zambryski P (2000). In: Buchanan BB, Gruissem W, Jones RL, (eds). Biochemistry and Molecular Biology of Plants. American Society of
Plant Physiologists, Rockville, MD; Pp. 988–1403.

 

Bewley JD,
Kent J, Bradford K, Hilhorst H and Nonogaki H (2013). Seeds: Physiology of Development,
Germination and Dormancy
, 3rd Edition. Springer New York; Pp. 1-399.       

 

Bino RJ, De-Vries JN, Kraak HL and Van-Pijlen JG (1992). Flow
cytometric determination of nuclear DNA replication stages in tomato seeds
during priming and germination. Annals of Botany; 69: 231-236.

 

Chrispeels MJ and Varner JE (1967).
Gibberellic acid-enhanced synthesis and release of α-amylase and ribonuclease
by isolated barley aleurone layers. Plant Physiol., 42: 398-406.

 

Finkelstein
RR (2004). The role of hormones during seed development and germination. In: Davies, P.J. (Ed.), Plant Hormones: Biosynthesis, Signal
transduction, Action
! The Netherlands, Kluwer Academic Publishers, Dordrecht.
Pp. 513–537.

 

Jacobsen JV, Higgins TJV and Zwar JA (1979).
Hormonal control of endosperm function during germination. In The Plant
Seed.
eds. I. Rubenstein, B. G. Gegenbach, R. L. Phillips and C. E. Green.
Academic Press, New York, pp. 241-262.

 

Jones RL (1973). Gibberellins: their
physiological role. Ann. Rev. Plant Physiol. 24: 571-598.

 

Jones RL and Armstrong JE (1971). Evidence for
osmotic regulation of hydrolytic enzyme production in germinating barley seeds.
Plant Physiol., 48: 137-142.

 

Jones RL and Jacobsen JV (1982). The role of
endoplasmic reticulum in the synthesis and transport of α-amylase in barley
aleurone layers. Planta 156: 421-432.

 

Mensah
SI and Agbagwa IO (2001). The responses of seeds of Capsicum frutescens
L. to the exogenous application of some growth promoters. J. Agric.
Biotechnol. Environ.,
3: 37-47.

 

Mensah
SI and Agbagwa IO (2004). Breaking dormancy in Gmelina arborea Roxb through treatment of seed with chemical
substance and alternating temperature. Journal
of Biological Research and Biotechnology;
2(1): 59 – 66.

 

Miransari
M and Smith DL (2009). Rhizobial lipochitooligossacharides and gibberellins
enhance barley (Hordeum vulgare L.)
seed germination. African Journal of
Biotechnology
, 8: 270-275.

 

Miyoshi
K and Sato T (1997). The effects of kinetin and gibberellin on the germination
of dehusked seeds of Indica and Japonica rice (Oryza sativa L.) under
anaerobic and aerobic conditions. Annals of Botany, 80: 479-483.

Nonogaki H, Bassel G and Bewly J
(2010). Germination still a mystery. Plant
Science,
179: 574-581.

 

Pinfield NJ and Stobart AK (1969).
Gibberellic-stimulated nucleic and metabolism in the cotyledons and embryonic axes of Coryllus avellane (L.)
seeds. New Phytol., 68: 993-999.

 

Singh S,
Singh P, Sanders DC and Wehner TC (2001). Germination of watermelon seeds at low
temperature. Cucurbit Genetics
Cooperative
; 24: 59-64.

 

Subedi
KD and Ma BL (2005). Seed priming does not improve corn yield in a humid
temperate environment. Agronomy Journal;
97: 211-218.

 

Varner JE and Ho DT (1976). The role of
hormones in the integration of seedling growth. In The Molecular Biology of
Hormone Action.
ed. J. Papaconstantinou. Academic Press, New      York. pp. 173-194.

 

Vishal B and Kumar PP (2018). Regulation of
seed germination and abiotic stresses by gibberellins and abscisic acid. Front.
Plant Sci.,
9: 838

 

Yanyan
LV, Qing MO, Alison AP and Yanrong W (2018). DNA replication during seed germination,
deterioration and its relation to vigor in alfalfa and white clover. Crop           Science;
58(3): 1393-1401.


Zeb
A, Khan A, Khan R and Shabbir F (2018). Effect of different concentrations of
kinetin on seed germination in tomato. International Journal of Agronomy and
Agricultural Research,
12(2): 1-8.

 

Cite this Article: Mensah, SI; Ejeagba, PO; Okonwu, K (2020).
Effects of GA3, BAP and KNO3 on the Germination and DNA Content of Cucumber (Cucumis sativus L.). Greener Journal of Agricultural Sciences
10(2): 57-62.

.

 

Leave a Reply

Your email address will not be published. Required fields are marked *