Tsombou Et Al

Tsombou et al

Greener Journal of Agricultural Sciences Vol. 6 (4), pp. 159-166, April 2016.

 ISSN: 2276-7770  

Research Paper

Manuscript Number: 032316068


(DOI: http://doi.org/10.15580/GJAS.2016.4.032316068)

 

Pre-treatment of Stored Dry Seeds of Aframomum melegueta in-vitro

 

Tsombou FM1,
Anjah GM1*, Antoine Mvondo Ze2

 

1Dept. of Plant
Biology, University of Dschang, P.O. Box 67 Dschang, Cameroon.

2Dept.
of Soil Science, University of Dschang, P.O. Box 222, Dschang, Cameroon.


Abstract

Background: The increase in the value of NTFPs
concerns: seeds, fruits, edible vegetable, mushrooms, fish and game. Of these
NWFPs, Aframomum species among which
is A. melegueta (Jujube), a
herbaceous, perennial classified in the family Zingiberaceae, class Monocotyledonae is over harvested in the wild.

 

Methods: This study was conducted with a view
to maximize the germination of seeds of Aframomum
melegueta
and eliminate the obstacle of integumentary inhibition after a
month of storage of these seeds. Physical pre-treatments were done using three
methods which included the steeping of seeds in cold tap water at 24, 48 and 72
hours respectively, soaking the seeds in boiling water for 5, 10 and 15
minutes, and chemical application by soaking the seeds in sulphuric acid at 20,
40 and 60 minutes respectively before being placed in the germination Petri
dishes. The optimal conditions for germination of the seeds were determined
which included the speed of germination, germination percentage and seeds
latency. The data collected were submitted to the Duncan test at 5%
significance threshold.

 

Results: The results indicated that
pre-treatment with sulphuric acid diluted to 50% significantly improved the
germination of A.melegueta seeds.
This pre-treatment resulted in a 100 percent germination of seeds. The latency
period was significantly shorter (6 days) obtained against 50 days in the
control group. The seed germination rate with this pre-treatment was 2.2
grains/day against 0.3grains/day in the control group. Other pre-treatments such
as cold tap water and boiling water had no significant effect on the
germination of these seeds.

 

Conclusion: At the end of the experiment, it was
observed that chemical pre-treatment on dry seeds of A. melengueta was the best.

 

Key Words: Conservation, Germination, Non Wood Forest Products,
Silvilculture, Pre-treatments.

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