Tsombou Et Al


Tsombou et al

Greener Journal of Agricultural Sciences Vol. 6 (4), pp. 159-166, April 2016.

 ISSN: 2276-7770  

Research Paper

Manuscript Number: 032316068

(DOI: http://doi.org/10.15580/GJAS.2016.4.032316068)


Pre-treatment of Stored Dry Seeds of Aframomum melegueta in-vitro


Tsombou FM1,
Anjah GM1*, Antoine Mvondo Ze2


1Dept. of Plant
Biology, University of Dschang, P.O. Box 67 Dschang, Cameroon.

of Soil Science, University of Dschang, P.O. Box 222, Dschang, Cameroon.


Background: The increase in the value of NTFPs
concerns: seeds, fruits, edible vegetable, mushrooms, fish and game. Of these
NWFPs, Aframomum species among which
is A. melegueta (Jujube), a
herbaceous, perennial classified in the family Zingiberaceae, class Monocotyledonae is over harvested in the wild.


Methods: This study was conducted with a view
to maximize the germination of seeds of Aframomum
and eliminate the obstacle of integumentary inhibition after a
month of storage of these seeds. Physical pre-treatments were done using three
methods which included the steeping of seeds in cold tap water at 24, 48 and 72
hours respectively, soaking the seeds in boiling water for 5, 10 and 15
minutes, and chemical application by soaking the seeds in sulphuric acid at 20,
40 and 60 minutes respectively before being placed in the germination Petri
dishes. The optimal conditions for germination of the seeds were determined
which included the speed of germination, germination percentage and seeds
latency. The data collected were submitted to the Duncan test at 5%
significance threshold.


Results: The results indicated that
pre-treatment with sulphuric acid diluted to 50% significantly improved the
germination of A.melegueta seeds.
This pre-treatment resulted in a 100 percent germination of seeds. The latency
period was significantly shorter (6 days) obtained against 50 days in the
control group. The seed germination rate with this pre-treatment was 2.2
grains/day against 0.3grains/day in the control group. Other pre-treatments such
as cold tap water and boiling water had no significant effect on the
germination of these seeds.


Conclusion: At the end of the experiment, it was
observed that chemical pre-treatment on dry seeds of A. melengueta was the best.


Key Words: Conservation, Germination, Non Wood Forest Products,
Silvilculture, Pre-treatments.

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Ahoton LE, Adgakpa BG, M’poFontiM’po and Akpo LE (2009). Effets des                                                                                                                                                         
                       prétraitements des
semences sur la germination de Prosopis
Tropicultura. 4. Pp. 233-238.


Amponsah J, Danso K and Elegba W
(2012). Enhancing germination of Aframomum
K. Schum.through Ex-vitro and In-vitro Propagation
Techniques.Resilience of agricultural systems against crises. Ghana. 10p.


Anjah GM, Nguetsop VF, Tsombou FM and Njoya MT (2015). Effect of field capacity of sacred
forest soils on regeneration of Aframomum
on Western Highlands in Cameroon. Journal of Horticulture and Forestry 7 (5): 142-148.


Aronu AJ, Nwachukwu OC and Adebayo O (2010).The effect of
pre-treatments on germination of seeds of Aframomum
in southern Nigeria. Forestry Research Institute of Nigeria,
Eastern Research Station, Umuahia,  Abia
State, Nigeria. Journal of Environmental
Issues and Agriculture in Developing Countries
2 (1): 6-12


Broekhoven G (1996). Non –timber forest
products: ecological and economic aspects of exploitation  in 
Colombia,  Ecuador  and 
Bolivia  IUCN,  Gland, 
Suisse  et  Cambridge, Royaume-Uni. Pp. 30.


Diawara D (2000). Les données statistiques sur les produits forestiers
non-ligneux en république de Guinée. Collecte et analyse de données pour
l’aménagement durable des Forêts – joindre les efforts nationaux et
internationaux. Programme de partenariat CE-FAO (1998-2001). Ligne budgétaire
forêt tropicale B7-6201/97-15/VIII/FOR projet GCP/INT/679/EC. Pp.32.


Eyob S, Appelgren M, Rohloff G, Tsegaye A and Messele G
(2008).Traditional and Medecinal uses
of essential oil composition of leaves and rhizomes of Aframomum corrima.Gansen Sourthern Ethiopia. Science Africa Global Botanic 74: 181-185.


Eyob S (2009). Promotion of seed
germination, subsequent seedling growth and in vitro propagation of korima Aframomum corrorima.
Journal of Medicinal Plants Research. 3(9) : 652-659.


FAO (2005). Communiqué “ la déforestation se poursuit à un rythme alarmant-
Nouveau Chiffres de la  FAO sur les
forêts mondiales ‘’ Rome. Pp. 2.


Fawa G (2014). Phénologie et modes de propagation de quatre essences
agroforestières locales dans les hautes savanes guinéennes (Adamaoua,
Cameroun). Thèse de Doctorat/ Ph.D. Université de Ngaounderé. Pp. 210. 


Jaouadi W, Hamrouni L, Souayeh N and Khouja LM (2010). Etude de la germination des grains d’Acacia tortilis sous différentes contraintes abiotiques. Biotechnol.Agron.
Soc. Environ
. 14 (4): 643- 652


Keshava CK, Krishnakumar G and Shenoy
HS (2014). Seed germination studies
on lophopetalumwightianumarn. – An
evergreen species of the Western Ghats. International
Journal of Plant, Animal and Environmental Sciences
. 4(3) : 502-507.


Niang-Diop F, Sambou B and Lykkem  A (2010). Contraintes de régénération naturelle
de Prosopis africana : facteurs
affectant la germination des graines. Institut des Sciences Anta Diop.
BP : 5005. Dakar Fanna, Sénégal. 4 (5). Pp. 1693-1705.


Nunez MR and Calvo L (2000). Effect of
high temperature on seed germination of Pinus
and Pinus halepensis.
Forest Ecology and Management, 131: 183-190.


Tabuna H (1999). Le Marché des Produits forestiers non Ligneux de l’Afrique
Centrale en France et en Belgique.   OCCASIONAL PAPER . Pp. 32.


Tsombou FM (2015). Les
applications sylvicoles dans la régénération d’Aframomummelegueta dans les sols de trois forets sacrées de l’ouest
Cameroun (Bamendjinda, Bamendjo et MbingMekoup). Thèse de Doctorat, Université
de Dschang. 200p.


Wamegni J  (1991). Etude de la phénologie, des fruits,
des graines et de la germination de Fagara
Mémoire d’Ingénieur des Eaux et Forêts à l’Université de
Dschang. Pp. 50. 


P (2000). Enquête  préliminaire sur les
produits forestiers non ligneux présents sur les marchés de Libreville (Gabon).
(eds). Les produits forestiers non ligneux en Afrique recherches actuelles pour
le développement Rome. Pp. 20.

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