Return to Issue
Full text – PDF
Full text – EPUB
4,810 total views, 6 views today
Vol. 11(2), pp. 172-180, 2021
Copyright ©2021, the copyright of this article is retained by the author(s)
Microbial Load and Resistance Pattern of Bacteria Organisms in the Gastrointestinal Tract of Household Rat
Egbagba J.E1; Owhe-Ureghe U.B2; Alex-Wele M.A3; Lawson S.D4
1 Department of Medical Microbiology, Federal Medical Centre Yenagoa, Nigeria.
3Department of Microbiology, Faculty of Science, Delta State University, Abraka, Nigeria.
3Deartment of Medical Microbiology, University of Port Harcourt Teaching Hospital Port Harcourt.
4Department of Medical Microbiology and Parasitology, Rivers State University, Port Harcourt, Nigeria.
Full Text: HTML;EPUB
Aim: This study investigates the microbial load of household rats and their resistance micro-organisms which are transmissible to man. This knowledge is useful for household rat disease burden management in our environment and the healthcare setting.
Method: This was cross-sectional descriptive and analysis of 200 trapped rats collected from 100 households in Abraka, Nigeria after consent were obtained
Results: The results from the study shows more bacteria load in the small intestine of household rats compared to the throat and large intestines. Bacteriodes fragilis and Escherichia coli were the predominant bacteria obtained, while Candida albicans and other Candida species were the most abundant fungi seen. More than two-thirds of the bacteria isolates were resistant to commonly used antibiotics such as Ampicillin, Penicillin, Trimetoprin-Sulphamethoxazole, and Ceftriaxone.
Conclusion: Households rats in our study indicate a high bacteria and fungi load burden with increased bacteria resistance. Reporting this zoonotic disease associated with these organisms, proper diagnosis and management are required to mitigate this potential source burden of disease-causing agents.
Dr Egbagba J.E
E-mail: drjohnegbagba@ yahoo.com
The house mouse (Mus musculus) infestation has been a worldwide problem for ages and are known reservoirs of a number of human diseases.1
Rodents are known agents of many human bacterial, viral, protozoa and fungal diseases.2,3,1 They have been linked with the risks and transmission of diseases such as Salmonellosis, Campylobacteriosis, Gastroenteritis, Candidiasis, rat bite fever, leptospirosis, plague, and murine typhus epidemic3,4. Additionally the presence of household rats have been associated with multidrug resistant organisms that are likely transmittable to man.4,3,5.
General agreement exist about the similarities of rat and human gut normal flora. These consist of facultative bacteria such as Streptococcus, Staphylococcus, Escherichia coli and other Enterobacteriacae, however, the anaerobic bacteria predominate especially in the large gut with bacteriodes fragilis, bifidobacterium and Lactobacilli.6,7, 8. Similarly, the predominant fungi which colonize the gut includes Candida albican, other candida species, Malassezia furfur and the filamentous fungus, Cladosporium.9,10.
Although various researches have looked at rat gut microbial composition in relation to human disease transmission, however few have looked at as an antimicrobial resistant organism that is possibly transmissible to man.
There are scanty studies about rat zoonotic diseases in our environment and most zoonotic diseases are under reported. This poses a health risk to the populace and difficulty in disease management. 11
This article therefore set out to determine the microbial load of household rats and its antibiotic resistance organism that are transferable to man. The outcome of this study could be useful for furtherance of rat zoonotic diseases management in our environment and in healthcare setting.
Study Design and sampling
This cross-sectional analytical study was carried out in Abraka community in Ethiope East local Government Area, Delta state, Nigeria. Abraka is a town in Delta central senatorial district of Delta State with a population estimate of 32,069 and home to the famous Delta State University, Abraka Nigeria. The populace apart from the University Staffs and students are chiefly agrarian.
Analysis were done between august to December 2020. Consent was obtained from the community leaders and the heard of each household where rat were obtained with the aid of mouse trap.
A total of 100 mouse were obtain from 20 households. Rats were trapped using mouse glue board, measuring 30 cm in length and 20 cm in width. After thorough visual inspection of the room to identify rodents’ tracks, droppings, the glue board was placed on their path. Pieces of crayfish or smoked fish were placed in the middle of the board as bait. The glue board was left overnight and trapped rodents were collected alive and taken to the laboratory for identification and microbiological analysis.
The study was approved by the Delta State University Ethical review Board.
Preparation and Analysis of samples
Each of the rat samples were picked from the trap using forceps. The skin was swabbed using a sterile swab stick. The rats were dissected using a dissecting kit to obtain the large and small intestine. The throat, small and large intestines was also swabbed using a sterile swab stick and transferred respectively into sterile mortar .and then crushed using a sterile pestle to homogenize the intestine. The homogenate (Ig) was taken into a sterile test tube for serial dilution, enumeration and plating protocol.12
The sample (1g) were collected into a test tube containing 9mls of distilled water. Serial dilutions was then carried out on 5 test tubes and the 10 and 1O dilution of both dilutions were collected and used for pour plate. The sterile swabs were then streaked plated onto sterilized Nutrient agar, McConkey agar and Saboroud Dextrose agar. Inoculated plates incubated at 37°C for 18-24hrs. After incubation in both aerobic and conditions, the colonies were counted using ‘Viable Plate count Method’ as enumerated by Eby Basiri ( Microbiology Biol. 275)13.
The biochemical tests carried out as described in Microbiology laboratory manual (2014)14. The tests includes gram stain, motility, citrate utilization test, Oxidase, indole, catalase, triple sugar iron agar (TSI), Bile esculin agar, and sugar fermentation test (lactose, glucose, sucrose, xylose, galactose, maltose, D-sorbitol, D-mannitol mannose). Antibiotic sensitivity tests were performed using the standard diffusion technique(CLSI 201715. Most commonly used antibiotics discs were used for sensitivity testing and includes Ampicilli, Penicilin, amoxicillin, Trimetoprin-Sulphamethoxazole, Gentamycin, Ceftriazone, Imipenem and Vancomycin .
We set out to determine the various bacterial and fungi organisms in the gastrointestinal tracts of household mice as well as their antibiotic resistance pattern.
The microbial load from different parts of the gut, shows that the small intestines of our sampled rats has more bacterial and fungal load in comparison to the throat and large intestines (Table 1)
Figure 1, shows the various bacteria organisms and relative percentage prevalence obtained from cultures after 24 hours of incubation. It is evident that Bacteroides fragilis and Escherichia coli has the highest percentage prevalence of 28% and 15% respectively. This is followed by Salmonella typhimurium (13%) and Staphylococcus aureus (9.6%).
As shown in figure 2, the predominant fungi species isolated includes Candida albican (35.3%) and other candida species(26.6%).
Table 2 show the relative number of each bacteria isolates, and their various percentage resistance to each antibiotics used in sensitivity testing. A total of 375 bacteria organisms were isolated. Of this, a total of 327 and 224 bacteria isolates were tested against ampicillin and tetracycline discs respectively. Notably, all 12 species isolated bacteria from culture are nearly 100% percent resistant to Ampicillin andpenicillin(Table 2). More than two-third of the isolates were also resistant toTetracycline, 224 (77.9%), Amoxicillin- Clavulanic acid, 128 (71.5%), Trimetoprin-Sulphamethoxazole, 207 (60.2%) and Ceftriaxone 343 (60.1%). However, most isolates teste were sensitive to Vancomycin 38 (5.8%), Clindamycin 96 (21.3%) and Amikacin 271 (45.5%).
Table 1: Microbial load from different parts of rats
Figure 1: Various bacteria species isolated from the gastrointestinal tracts of household rats in Abraka community
Figure 2: Various types of fungi isolates from the gastrointestinal tracts of household rats in Abraka community
Table 2: Antimicrobial resistance pattern of bacteria isolates from cultured plates in the study
In recent years, there exist a growing concern about the increase in zoonotic diseases transmission and emergence of resistance organism between house hold rats and human2. This indicate risk associated frequent contact between human, household and other human pets.
The result obtained from this investigation shows an abundant number of bacteria and fungi organisms in the gut of household rats that are possibly transmissible to man. It also shows that over two-third of the bacteria organisms isolated were resistant to commonly used antibiotics such as Ampicillin, Penicillin, Tetracycline, Trimethoprim- Suphamethoxazole and Ceftriaxone. The gut microbial population ( Table 1) consists of bacteria and fungi which may be symbiotic, neutral or harmful to the host.16 These microbiota are important for the host immune response to diseases and maintenance of healthy state 16. Possible mechanism for abundant microbial load includes genetics, diets and feeding habits.17,18 The abundant microbes in our results is consistent with report of Li Wen et al (2017), which shows that gut microbes increases significantly from the stomach, jejunum to large intestines. The large gut harbours up to 1000 different bacterial and additional 1 x 1014 different organisms.16,
The largest gut bacteria flora in this study were bacteriodes fragilis followed by Escerichia coli and Salmonella typhimurium respectively. Similar results have been reported by Geraldine O. Canny et al (2008) who reported bacteriodes fragilis as the largest gut flora. This result is in contrast to result presented by Tomotari Mitsuoka (1992), who reported that lactobacilli actually predominate in rat and human intestines.7,8. Possible speculation to differences in gut microbes could be due to the breeding, diets and environmental condition of the host19,1.17
Fungi isolates which dominated our study were candida albicans, and other candida species. This shows that fungi are also part of the normal flora of household rats as in human. These results are in agreement with previous studies which shows that candida and other fungi species are part of the rat gut ubiquitous microbiota and also serves as either commensals, pathogens or opportunistic organisms.20,21,22.
Perhaps, the most clinically relevant finding in this study is the carriage of antibiotic resistant organisms by these rats (Table 2). As a result of this carriage, transmission of resistant organisms to humans are highly probable through a variety of routes. These routes includes direct contact, the food chain, (where rats are part of the household diets or come into direct contact with food) and contact with direct surfaces.2,23, 24This can lead to the advent of difficult-to-manage diseases.24, 25.
Household rats have a closer relationship with humans and they have sought a niche role in human-occupied structures such as houses, classrooms, and restaurants. This, along with other urban rats, are found to host antimicrobial-resistant bacteria such as E. coli and S. aureus, which can be readily spread to humans by (1) direct or near interaction and (2) ingestion of utensils and fruit. Non-hygienic food handling will escalate this.11
House-holds rat ability to act as reservoirs and vectors of antimicrobial resistance suggests that they could serve as sentinels for the occurrence, transmission, and potential human health risks of antimicrobial resistance2,26,27.
Possible mechanisms for antibiotic resistance includes; (1) the presence of antimicrobial resistance genes (intrinsic resistance), (2) the selective pressure exerted by excessive antibiotic use by humans.28,29.
Our study implies that over 66%of bacteria isolates were resistant to commonly used antibiotic. This results is consistence with reports by Alermale Admas et al (2020), Melkanu Abebe Abebe et al (2019), Richard J. Fair and Yithak Tor (2014).30,31,32 In contrast to our study, a lower percentage of 44.4% of total isolated organisms being resistant to commonly used antibiotics has been reported.33Speculated reasons for differences in antibiotic resistance pattern could due to techniques, equipment used for antibiotics resistant testing, rational use of antibiotics, and local resistant pattern.34
Of the 38 isolates that were tested against Vancomycin, an average of 3.8% were resistant. Rodriques J et al (2002) reported a lower average vancomycin resistance of 0.0% while higher percentages of 15 to 30% have been reported by other several other studies. 34,31,35,36,37.
There are little or scanty studies on the burden of organisms in the gut of house rats and its impact on house hold dwellers in our environment38. This study therefore serve as baseline study and may be useful for Health Practitioners, environmental impact assessment officers and policy makers. Household rats have long been a concern in rural areas, eating and contaminating stored food, spreading disease, and degrading the physical environment.39,11,27
The underreporting of rats zoonoses and, in many cases, inadequate attention paid to the diagnosis and management of these essential diseases are two main messages to emerge from this study.3, 5
When exploring ways to minimize the impact of rodents on rural livelihoods, there are two key issues to consider: 1) recognizing the current impact of rodents on food protection, health, and nutrition of communities, as well as existing awareness, behaviour, and practices of people regarding their rodent problems, and 2) designing cost-effective strategies that can be sustained.38
The results of this study should be interpreted with caution as it was done in a small community in Abraka, Delta State, Nigeria. This therefore limits its generalization. Our techniques of disk diffusion as methods of resistance testing could also be prone to technical and observer’s error. Antifungi resistance testing were not included in our study due paucity of equipment. More extensive studies on burden of household rat borne pathogens using modern techniques are required in our environment.
Taken together, the result of this study indicates a high burden of bacteria, fungi and bacteria resistance organisms in household rats that can be transmitted to man.
It also suggest that increased reporting of this zoonotic disease coupled withp roper diagnosis and management are ways of mitigating this burden.
Egbagba J.E: Designed the study, protocol writing, manuscript preparation, statistical analysis,
Alex-Wele M.A: sample collection, manuscript preparation:
Lawson S.D: Data collection and Statistical analysis
Owhe Ureghe U.B: Laboratory analysis and Supervision
All authors read and approved the final manuscript
We are grateful to the members of the Department of Microbiology,
Faculty of Science, Delta State University, Abraka. We also thank members of Abraka community for giving us consent to collect rats from their various households
Funding: No funding sources
Conflict of interest: None declared
Ethical clearance for the study was granted by the Ethics
Review Board of Delta State University, Abraka.
1. Jahan NA, Lindsey LL, Larsen PA. The Role of Peridomestic Rodents as Reservoirs for Zoonotic Foodborne Pathogens. doi:10.1089/vbz.2020.2640
2. Gwenzi W, Chaukura N, Muisa-Zikali N, et al. Insects, rodents, and pets as reservoirs, vectors, and sentinels of antimicrobial resistance. Antibiotics. 2021;10(1):1-42. doi:10.3390/antibiotics10010068
3. Meerburg BG, Singleton GR, Kijlstra A. Rodent-borne diseases and their risks for public health Rodent-borne diseases and their risks for public health. Crit Rev Microbiol. 2009;35(3):221-270. doi:10.1080/10408410902989837
4. Fornefett J, Busch A, Döpping S, Hotzel H, Rimek D. Bacterial gastroenteritis caused by the putative zoonotic pathogen Campylobacter lanienae: First reported case in Germany. Access Microbiol. 2021;3(3). doi:10.1099/acmi.0.000199
5. Meerburg BG, Kijlstra A. Role of rodents in transmission ofSalmonella andCampylobacter. J Sci Food Agric. 2007;87(15):2774-2781. doi:10.1002/jsfa.3004
6. Inoue R, Ushida K. Development of the intestinal microbiota in rats and its possible interactions with the evolution of the luminal IgA in the intestine. FEMS Microbiol Ecol. 2003;45(2):147-153. doi:10.1016/S0168-6496(03)00134-X
7. Canny GO, McCormick BA. Bacteria in the intestine, helpful residents or enemies from within? Infect Immun. 2008;76(8):3360-3373. doi:10.1128/IAI.00187-08
8. Mitsuoka T. The Human Gastrointestinal Tract. In: The Lactic Acid Bacteria Volume 1. Springer US; 1992:69-114. doi:10.1007/978-1-4615-3522-5_4
9. Hallen-Adams HE, Suhr MJ. Fungi in the healthy human gastrointestinal tract. Virulence. 2017;8(3):352. doi:10.1080/21505594.2016.1247140
10. Hof H. Pilze im Darm – das Mykobiom des Darms. Z Gastroenterol. 2017;55(8):772-778. doi:10.1055/s-0043-112657
11. Gwenzi W, Chaukura N, Muisa-Zikali N, et al. Insects, rodents, and pets as reservoirs, vectors, and sentinels of antimicrobial resistance. Antibiotics. 2021;10(1):1-42. doi:10.3390/antibiotics10010068
12. Serial Dilutions and Plating: Microbial Enumeration | Protocol. Accessed May 14, 2021. https://www.jove.com/v/10507/serial-dilutions-and-plating-microbial-enumeration
13. Bassiri E. Microbiology BIOL 275.
14. (PDF) Microbiology Laboratory Manual. Accessed May 14, 2021. https://www.researchgate.net/publication/306018042_Microbiology_Laboratory_Manual
15. clsi 2017 pdf – Google Search. Accessed May 14, 2021. https://www.google.com/search?q=clsi+2017+pdf&oq=clsi+2017&aqs=chrome.1.69i57j0l3j0i22i30l6.6682j0j15&sourceid=chrome&ie=UTF-8
16. Fujimura KE, Slusher NA, Cabana MD, Lynch S V. Role of the gut microbiota in defining human health. Expert Rev Anti Infect Ther. 2010;8(4):435-454. doi:10.1586/eri.10.14
17. Wen L, Duffy A. Factors influencing the gut microbiota, inflammation, and type 2 diabetes. J Nutr. 2017;147(7):1468S-1475S. doi:10.3945/jn.116.240754
18. Stringer AM, Gibson RJ, Logan RM, et al. Gastrointestinal microflora and mucins may play a critical role in the development of 5-fluorouracil-induced gastrointestinal mucositis. Exp Biol Med. 2009;234(4):430-441. doi:10.3181/0810-RM-301
19. Tomas J, Langella P, Cherbuy C. The intestinal microbiota in the rat model: major breakthroughs from new technologies. Anim Health Res Rev. 2012;13(1):54-63. doi:10.1017/S1466252312000072
20. Fidel PL. Candida albicans: from commensal to pathogen. In: Medical Importance of the Normal Microflora. Springer US; 1999:441-476. doi:10.1007/978-1-4757-3021-0_18
21. Böhm L, Torsin S, Tint SH, Eckstein MT, Ludwig T, Pérez JC. The yeast form of the fungus Candida albicans promotes persistence in the gut of gnotobiotic mice. PLoS Pathog. 2017;13(10). doi:10.1371/journal.ppat.1006699
22. Basmaciyan L, Bon F, Paradis T, Lapaquette P, Dalle F. “Candida Albicans Interactions With The Host: Crossing The Intestinal Epithelial Barrier.” Tissue Barriers. 2019;7(2). doi:10.1080/21688370.2019.1612661
23. Huff R, Pereira RI, Pissetti C, et al. Antimicrobial resistance and genetic relationships of enterococci from siblings and non-siblings Heliconius erato phyllis caterpillars. PeerJ. 2020;2020(2). doi:10.7717/peerj.8647
24. Pietri JE. Case not closed: Arguments for new studies of the interactions between bed bugs and human pathogens. Am J Trop Med Hyg. 2020;103(2):619-624. doi:10.4269/ajtmh.20-0121
25. Kruse H, Sørum H. Transfer of multiple drug resistance plasmids between bacteria of diverse origins in natural microenvironments. Appl Environ Microbiol. 1994;60(11).
26. Kolawole OM, Anjorin EO, Adekanle DA, Kolawole CF, Durowade KA. Seroprevalence of rubella IgG antibody in pregnant women in osogbo, Nigeria. Int J Prev Med. 2014;5(3):287-292. Accessed December 5, 2020. /pmc/articles/PMC4018637/?report=abstract
27. Zhong XS, Li YZ, Ge J, et al. Comparisons of microbiological characteristics and antibiotic resistance of Klebsiella pneumoniae isolates from urban rodents, shrews, and healthy people. BMC Microbiol. 2020;20(1). doi:10.1186/s12866-020-1702-5
28. C Reygaert W. An overview of the antimicrobial resistance mechanisms of bacteria. AIMS Microbiol. 2018;4(3):482-501. doi:10.3934/microbiol.2018.3.482
29. Davies J, Davies D. Origins and Evolution of Antibiotic Resistance. Microbiol Mol Biol Rev. 2010;74(3):1092-2172. doi:10.1128/MMBR.00016-10
30. Admas A, Gelaw B, Belaytessema, Worku A, Melese A. Proportion of bacterial isolates, their antimicrobial susceptibility profile and factors associated with puerperal sepsis among post-partum/aborted women at a referral Hospital in Bahir Dar, Northwest Ethiopia. Antimicrob Resist Infect Control. 2020;9(1). doi:10.1186/s13756-019-0676-2
31. Abebe M, Tadesse S, Meseret G, Derbie A. Type of bacterial isolates and antimicrobial resistance profile from different clinical samples at a Referral Hospital, Northwest Ethiopia: Five years data analysis. BMC Res Notes. 2019;12(1). doi:10.1186/s13104-019-4604-6
32. Fair RJ, Tor Y. Antibiotics and bacterial resistance in the 21st century. Perspect Medicin Chem. 2014;6(6):25-64. doi:10.4137/PMC.S14459
33. Nigussie D, Makonnen E, Legesse BA, Fekadu A, Davey G. Antimicrobial susceptibility of bacteria isolated from the infected wounds of patients with lymphoedema in East Wollega, Ethiopia. Trans R Soc Trop Med Hyg. 2020;114(12):962-973. doi:10.1093/trstmh/traa143
34. Plaza-Rodríguez C, Alt K, Grobbel M, et al. Wildlife as Sentinels of Antimicrobial Resistance in Germany? Front Vet Sci. 2021;7. doi:10.3389/fvets.2020.627821
35. Vera-Lise Tulstrup M, Gerd Christensen E, Carvalho V, et al. Antibiotic Treatment Affects Intestinal Permeability and Gut Microbial Composition in Wistar Rats Dependent on Antibiotic Class. Published online 2015. doi:10.1371/journal.pone.0144854
36. Faron ML, Ledeboer NA, Buchan BW. Resistance mechanisms, epidemiology, and approaches to screening for vancomycin-resistant Enterococcus in the health care setting. J Clin Microbiol. 2016;54(10):2436-2447. doi:10.1128/JCM.00211-16
37. Fridkin SK, Edwards JR, Courval JM, et al. The effect of vancomycin and third-generation cephalosporins on prevalence of vancomycin-resistant enterococci in 126 U.S. adult intensive care units. Ann Intern Med. 2001;135(3):175-183. doi:10.7326/0003-4819-135-3-200108070-00009
38. Belmain SR, Meyer A, Partnership A, et al. Assessment of the Impact of Rodents on Rural Household Food Security and the Development of Ecologically-Based Rodent Management Strategies in Zambézia Province, Mozambique Developing Rodent Management Strategies for Rural Households in Mozambique.; 1999.
39. Özkara A, Akyil D, Konuk M. Pesticides, Environmental Pollution, and Health. In: Environmental Health Risk – Hazardous Factors to Living Species. InTech; 2016. doi:10.5772/63094
Download [498.16 KB]
4,808 total views, 4 views today
Your email address will not be published. Required fields are marked *
Save my name, email, and website in this browser for the next time I comment.