Molecular Detection and Antibiotic Susceptibility Profile of ESBL-producing Klebsiella pneumoniae Isolates in a Central Nigerian Tertiary Hospital

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Greener Journal of Medical Sciences

Vol. 11(2), pp. 181-186, 2021

ISSN: 2276-7797

Copyright ©2021, the copyright of this article is retained by the author(s)

https://gjournals.org/GJMS

Molecular Detection and Antibiotic Susceptibility Profile of ESBL-producing Klebsiella pneumoniae Isolates in a Central Nigerian Tertiary Hospital

Enyinnaya S.O1*, Iregbu K.C2, Uwaezuoke N.S3, Abdullahi N3, Lawson S.D1

1Department of Medical Microbiology and Parasitology, Faculty of Basic Clinical Sciences, Rivers State University, Port Harcourt, Rivers State, Nigeria.

2Department of Medical Microbiology and Parasitology, National Hospital, Abuja.

3 Department of Medical Microbiology and Parasitology, Federal Medical Centre, Abuja.

ARTICLE INFO ABSTRACT
Article No.: 110521113

Type: Research

Full Text: HTML;EPUB

Background: Production of extended-spectrum β-lactamases (ESBLs) is the most common mechanism of resistance to third-generation cephalosporins among Enterobacteriaceae including Klebsiella pneumonia and this presents therapeutic challenges managing infections caused by these strains of bacteria.

Aim: To determine the prevalence, antibiotic susceptibility profile and major ESBL encoding genes among Klebsiella pneumoniae in clinical specimens.

Methods: Four hundred (400) consecutive and non-duplicate isolates of Klebsiella pneumoniae from clinical specimens were identified by standard laboratory methods at the National Hospital Abuja, subjected to antimicrobial susceptibility testing using the Kirby-Bauer disc diffusion method and identified ESBL phenotypes were confirmed using E-test. Multiplex PCR was used to detect ESBL genes.

Results: Out of the 400 Klebsiella pneumoniae isolates, 114 (28.5%) were ESBL producers, out of which 111 (97.4%) were sensitive to meropenem, 101 (88.6%) to amikacin, 100 (87.7%) to fosfomycin, 96 (84.2%) to tigecycline, and 58 (50.9%) to nitofurantoin. All the ESBL producers were resistant to cefotaxime while 107 (93.9%) and 105 (92.1%) were resistant to amoxicillin-clavulanate, and ceftazidime respectively . There was a significantly higher distribution of multidrug resistance among ESBL producing isolates compared to non-ESBL producing isolates (chi-square =63.29, p-value = 0.0001). The distribution showed that 78 (70.3%) had the blaSHV gene, 99 (89.2%) had the blaCTX-M gene, 88 (79.3%) had the blaTEM gene and 3 (2.6%) had none of the major bla genes.

Conclusion: This study showed a relatively high prevalence of ESBL-producing Klebsiella pneumoniae isolates and a significant occurrence of multidrug-resistant Klebsiella pnuemoniae. Meropenem and amikacin are excellent therapeutic choices for empirical therapy of ESBL-producing Klebsiella pneumoniae infections and their use should be properly guarded through efficient infection control and antimicrobial stewardship..

Accepted: 05/11/2021

Published: 15/11/2021

*Corresponding Author

Enyinnaya, SO

E-mail: stellaoikedichi@ gmail.com

Keywords: Klebsiella pnuemoniae; ESBL; Multiplex-PCR; Antibiotic Resistance.
   

INTRODUCTION

Extended spectrum beta-lactamase (ESBL) producing Klebsiella pneumoniae was first reported in Germany in 1983 with subsequent increased global reporting over the decades1. It was later reported in Escherichia coli, Pseudomonas aeruginosa and other gram-negative bacilli2. ESBLs are a large, rapidly evolving group of plasmid-mediated enzymes that confer resistance to the oxyimino cephalosporins and monobactams but not to cephamycins or carbapenems, and are inhibited by β-lactamase inhibitors such as clavulanic acid3. They are the first example in which β-lactamase–mediated resistance to β-lactam antibiotics resulted from fundamental changes in the substrate spectra of the enzymes4.  ESBL producing Klebsiella pneumoniae is among the commonest Gram-negative bacilli implicated in community- and hospital-acquired infection5–7, causing intra-abdominal infection, urinary tract infection, respiratory infection and sepsis8. Infections caused by ESBL producing Gram-negative bacteria, including K. pneumoniae are associated with severe adverse outcomes, including higher overall and infection-related mortality, increased length of hospital stay, discharge to chronic care, and higher costs9

Production of extended-spectrum β-lactamases (ESBLs) is the most common mechanism of resistance to third-generation cephalosporins among Enterobacteriaceae, including Klebsiella pneumoniae and Escherichia coli. ESBL-producing K. pneumoniae usually express multidrug resistance and the spread of these multidrug-resistant bacteria has become a public health concern on a global scale, and particularly affects low- and middle-income countries10. These strains of bacteria also have the capacity to acquire resistance to other antimicrobial classes such as the quinolones, tetracyclines, cotrimoxazole, trimethoprim, and aminoglycosides, in addition to the penicillins, cephalosporins and aztreonam, and this further limits therapeutic options11–13

It has been established that epidemiological data on multidrug-resistant organisms in sub-Saharan Africa are scarce 14. A recent systematic review revealed that the lack of data on the occurrence of multidrug-resistant Gram-negative bacteria, including Klebsiella pneumoniae, is greatest in West Africa15. This situation applies to Abuja, Nigeria where this study was carried out; previous study on ESBL-producing K. pneumoniae are scanty, thus leaving a huge knowledge gap which this study aims to bridge. Knowledge of the magnitude and antimicrobial susceptibility profile of ESBL producing K.pneumoniae will go a long way in assisting clinicians optimise antimicrobial therapy with improved patient outcomes.

METHODS

Study Design and Area

This was a cross-sectional study carried out in the Department of Medical Microbiology and Parasitology, National Hospital Abuja, a 500-bed tertiary hospital located in the Federal Capital Territory, Abuja. It is a referral centre for the federal capital territory and neighbouring states of Nigeria, providing health care services to about 50,000 patients monthly. The study involved 400 K pneumoniae isolated from consecutively selected clinical specimens from blood, urine, wound biopsy/swab, cerebrospinal fluid (CSF), sputum, aspirates, ear and eye swabs.

Isolation and Identification of K pneumoniae

All samples were first inoculated on blood agar, MacConkey agar, and CLED agar (for urine samples) plates and incubated at 350C for 24 hours in ambient air. Lactose-fermenting, convex, entire edge, large, mucoid colonies that were gram-negative short bacilli , non-motile, indole negative, methyl red negative, voges prausker positive, citrate-positive, and urease-positive were identified as K. pneumoniae following established procedures16,17 .

Antibiotic susceptibility testing

Antimicrobial susceptibility was done by the disc diffusion method using the modified Kirby-Bauer method.18 The susceptibility of all isolates was tested to the following antimicrobial agents: Ampicillin (10µg), Amoxicillin/Clavulanic acid (20/10µg), Cefuroxime (30µg), Meropenem (10µg), Chloramphenicol(30µg), Gentamicin (10µg), Ciprofloxacin (5µg), Amikacin (30µg), Fosfomycin (200µg), Tigecycline (30µg), Nitrofurantoin (300µg) according to CLSI guideline.19 These antibiotics were selected according to previously published recommendations and their widespread use in treatment of various diseases.18 E-test (Liofilchem Diagnostics, Abruzzi, Italy) for confirming the ESBL phenotype was performed according to manufacturer’s guidelines. ESBL results were considered positive if the isolates had an MIC (µg/ml) of ≥1 for ceftazidime (CAZ), ≥0.5 for cefotaxime (CTX), and the ratio for ceftazidine/ceftazidine +clavulanic acid (CAZ-CLA) and cefotaxime/cefotaxime+clavulanic acid (CTX-CTL) was more than or equal to 8 .20

Molecular Characterization

Multiplex PCR was performed in a single tube with primers of blaTEM, blaSHV, blaOXA and 16S rRNA genes. PCR assay was performed in a total volume of 50 µl which contained; 25 pmol of the primers of 16S rRNA (Fd 5′-TGTGGGAACGGCGAGTCGGAATAC-3′ and Rev 5′-GGGCGCAGGGGATGAAACTCAAC-3′), 10 pmol primers of each of the blaTEM, blaSHV, and blaOXA as described by Trung et al.21 200 µM each of the dNTPs, 1U of Taq DNA polymerase, 1×PCR assay buffer with 1.5 mM MgCl2 and 100 ng of template DNA. PCR conditions were used as described by Trung et al.21 PCR was run in a PTC-100 Thermal Cycler (MJ Research, Inc., USA). 5 µl of the amplified PCR product was used for electrophoresis and visualization was done with a UV transilluminator. Amplified productsof blaTEM, blaSHV, blaOXA, and blaCTX-M genes were purified by QIAquick gel extraction kit (Qiagen, Hilden, Germany) according to the instructions of the manufacturer. Positive and negative controls were used

Data Collection

Data of all the K. pneumoniae isolates from the various specimens, their antibiotic susceptibility testing, phenotypically confirmed ESBL strains as well as their responsible ESBL genes detected via molecular method were collected.

Data Analysis

All data collected was analysed using the software statistical package for social sciences (SPSS) version 25 by IBM SPSS Statistics. Percentage prevalence of ESBL and non- ESBL isolates, multidrug resistance among non ESBL and ESBL isolates and other results were presented using tables and charts. All analyses were done at a 95% confidence interval and a p value of < 0.05 was considered statistically significant.

Ethical approval

Ethical approval was obtained from the Health Research Ethics Committee (HREC) of National Hospital Abuja.

RESULTS

Findings showed that 114 (28.5%) out of the 400 Klebsiella pneumoniae isolated were ESBL producers.

Table 1 shows the susceptibility pattern of all the four hundred isolates. Among the 114 ESBL producing isolates, 111 (97.4%) were sensitive to meropenem, 100 (87.7%) to fosfomycin, 98 (84.2%) to tigecycline, 101 (88.6%) to amikacin, and 58 (50.9%) to nitofurantoin. only 2 (1.8%) to ceftazidine. All the 114 ESBL producers were resistant to ceftriaxone while 107 (93.9%) and 105 (92.1%) were resistant to amoxicillin-clavulanate and ceftazidime respectively. Table 2 shows a significantly higher distribution of multidrug resistance among ESBL producing isolates compared to non-ESBL producing isolates (chi-square =63.29, p= 0.0001). Distribution of the bla genes among the Klebsiella pneumoniae isolates showed that 78 (70.3%) had the blaSHV gene, 99 (89.2%) had the blaCTX-M gene, 88 (79.3%) had the blaTEM gene and 3 (2.6%) had no bla genes as shown in table 3.

Table 1: Antibiotic susceptibility pattern of K. pneumoniae isolates

Antibiotics ESBL Producing K. pneumoniae Non-ESBL producing K. pneumoniae
S I R S I R
Amikacin 101 (88.6) 2 (1.8) 11 (9.7) 272 (95.1) 14 (4.9)
Amoxicillin-clavulanate 1 (0.9) 6 (5.3) 107 (93.9) 71 (24.8) 65 (22.7) 150 (52.5)
Ampicillin 114 (100) 286 (100)
Chloramphenicol 56 (49.1) 58 (50.9) 199 (69.6) 31 (10.9) 56 (19.6)
Ceftazidime 2 (1.8) 7 (6.1) 105 (92.1) 286 (100)
Ciprofloxacin 9 (7.9) 4 (3.5) 101 (88.6) 103 (36) 111 (38.8) 72 (25.2)
Gentamicin 5 (4.4) 109 (95.6) 206 (72) 2 (0.7) 78 (27.3)
Cefotaxime 114 (100) 286 (100)
Cefuroxime 2 (1.8) 112 (98.3) 262 (91.6) 10 (3.5) 14 (4.9)
Nitrofurantoin 58 (50.9) 4 (3.5) 52 (45.6) 202 (70.6) 10 (3.5) 74 (25.9)
Fosfomycin 100 (87.7) 2 (1.8) 12 (10.5) 282 (98.6) 4 (1.4)
Meropenem 111 (97.4) 3 (2.6) 286 (100)
Tigecycline 96 (84.2) 15 (13.2) 3 (2.6) 240 (83.9) 38 (13.3) 8 (2.8)

S: Sensitive, I: Intermediate, R: Resistant

Table 2: Distribution of Multidrug Resistant isolates among K. pneumoniae

Multidrug Resistance ESBL Chi-square

(p-value)

Yes (n, %) No (n, %)
Yes 114 (100.0) 147 (51.40) 63.29 (0.0001)*
No 0 (0.0) 139 (48.60)
Total 114 (100.0) 286 (100.0)  

*Distribution is statistically significant (p < 0.005).

Table 3: Distribution of bla genes among ESBL-Klebsiella pnuemoniae isolates

Genes Frequency n =114 (%)
blaCTX-M alone 12 (10.8)
blaTEM alone 6 (5.4)
blaSHV +bla CTX-M 11 (9.9)
blaSHV +bla TEM 6 (5.4)
blaCTX-M +bla TEM 15 (13.5)
blaSHV+blaCTX-M+blaTEM 61 (55.0)
None of the major bla 3(2.6)
Total CTX-M 99 (89.2)
Total TEM 88 (79.3)
Total SHV 78 (70.3)

DISCUSSION

The study showed a 28.5% prevalence of ESBL-producing Klebsiella pneumoniae among the isolates, and compares well with the 30% 22 and 31.6% 23 reported by Raji et al in Lagos, but differs widely from 60.8% 24 reported by Aibinu et al in Lagos. The relatively lower prevalence of ESBL-producing K. pneumoniae observed in the current study could be attributed to the variation in antibiotic use, sensitivity and specificity of test methods compared to the other study sites of the aforementioned studies.The high rate of multidrug resistance among both ESBL and Non-ESBL producing K.pneumoniae found in this study has similarly been reported from studies done in Kano25 and Ibadan26. This could be due to previous exposure to antibiotics resulting from self medication that is widely prevalent in this part of the world due to easy access and purchase of antibiotics across the counter for use without prescription. This has led to major challenge in the therapeutic management of serious life threatening infections due to these strains with poor outcome.26,27 It is particularly noteworthy that about 81% of all blood isolates were ESBL-producers, technically eliminating the third generation cephalosporins as empiric treatment for blood stream infections.

Notwithstanding the observed high rate of multidrug resistance, all the ESBL-producing K. pneumoniae isolates had relatively high rate of susceptibility to meropenem, amikacin, fosfomycin, and tigecycline, serving as safety valves and giving some hope of rescue when the third generation cephalosporins fail. When considered against the background of high ESBL prevalence, meropenem obviously stands out as the drug of first choice for empiric treatment in serious life-threatening infections. Amikacin would appear to be an alternative empiric therapy drug based on susceptibility and cost, but its choice may be limited by its side effect especially in neonates .28 The use of tigecycline, an antibiotic recently introduced into the hospital and used mainly in the burns unit for non-pseudomonal infections, when indicated, is limited to skin and soft tissue infections29.

In this study, the predominant gene was blaCTX-M and it exists alone or in association with other genes, TEM and SHV, but predominantly with TEM genes. This very high prevalence of blaCTX-M type ESBL genes among K.pneumoniae isolates supports the worldwide pandemic spread of the CTX-M β-lactamase enzyme as reported in America30,Europe31, Middle east 32,Asia 33 and Africa34. Studies in Nigeria by Iroha et al 35 , Raji et al 23 and Aibinu et al36 also lend credence to the high prevalence of blaCTX-M type. ESBL mediated by blaCTX-M type β-lactamase genes are undoubtedly the most widespread enzymes produced among K. pneumoniae.

The large proportion of the ESBL producers that harboured multi-genes in this study is worrisome and may partly explain the observed high level of drug resistance, even in the presence of β-lactamase inhibitors. It is likely these isolates hyper produce β–lactamase enzymes which overwhelm the β–lactamase inhibitors 37. The carriage of these genes on plasmids enhances the spread and therefore requires good infection control measures to limit dissemination.

CONCLUSION

ESBL producing K. pneumoniae strains are relatively high among K. pneumoniae isolates. The isolates were significantly associated with multi-drug resistance. Interestingly, both the ESBL and non-ESBL strains were highly sensitive to meropenem amikacin, fosomycin, and tigecycline. The predominant ESBL gene among K. pneumoniae isolates was blaCTX-M , and a significant proportion of the ESBL isolates harboured 2 or 3 ESBL genes together. This study highlights the need for efficient infection control and antibiotics stewardship practices to mitigate the rising cases of antimicrobial resistance. The remarkable difference in sensitivities between ESBL-producing and non-ESBL-producing isolates makes it imperative to test for ESBL-production routinely

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Cite this Article: Enyinnaya SO; Iregbu KC; Uwaezuoke, NS; Abdullahi, N; Lawson, SD (2021). Molecular Detection and Antibiotic Susceptibility Profile of ESBL-producing Klebsiella pneumoniae Isolates in a Central Nigerian Tertiary Hospital. Greener Journal of Medical Sciences, 11(2): 181-186.

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