Vol. 4(1), pp. 1-6, 2019
ISSN: 2354-2284
Copyright ©2018, the copyright of this article is retained by the author(s)
DOI Link: http://doi.org/10.15580/GJMA.2019.1.120918168
http://gjournals.org/GJMA
Isolation and Identification of Microorganisms Encountered in Traditional Fermentation of Soya Beans and Roselle seeds for the production of Food Tasty condiment (Dawadawa)
1&2Adamu, Shahidah A.; 2Farouq, A. A.; 3Magashi, M. A.; 4Sokoto, A. M.; *5Salau, I. A.
Article No.: 120918168
Type: Research
DOI: 10.15580/GJMA.2019.1.120918168
Study on isolation and identification of microorganisms encountered in traditional fermentation of Soya Beans and Roselle seeds for the production of Dawadawa was carried out, using standard microbiological and biochemical methods. The organisms identified are from genera Bacillus, Kurthia, Staphylococcus, Listeria and Micrococcus. Yeast and fungi were also isolated. Hazard and critical control point (HACCP) at each stage during fermentation was determined, washed seeds and water used for washing was found to have high number of microorganisms. Microbial fermentation increased the bioavailability of nutrients in the condiments produced from the seeds of G. max and H. sabdariffa.
Submitted: 09/12/2018
Accepted: 13/12/2018
Published: 06/01/2019
*Corresponding Author
Ibrahim A. Salau
E-mail: ibrahimasalau@ gmail.com
Phone: +234(0)8053569551
Keywords:
Microorganisms; Soybeans; Roselle seeds; Dawadawa; Traditional fermentation Return to Content View [Full Article – PDF]
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REFERENCES
Achi OK. (1992). Microorganisms associated with natural fermentation of Prosopis africana seeds for the production of Okpiye Plant Foods. Human. Nutrition 42: 297-304.
Adewumi BA and Olalusi, AP (1995): Performance of a Manual operated concave-type Locust Bean Dehuller. Journal of Agricultural. Technology 2:23:30.
Adewusi SRA, Orisadare BO and Oke OL (1991). Studies on wearing Diet in nigeria, 1. Carbohydrate source. Cereal chemistry., 165-169
Adewusi SRA, Orisadare BO and Oke OL (1992). Studies on wearing Diet in nigeria, 2. Protein source. Plant food for Human Nutrition, 42: 183-192.
Antai SP and Ibrahim HM (1986) Microorganisms associated with African locust bean (Parkia filicoidea Welw) fermentation for dawadawa production . Journal of applied bacteriology, 61:145-148
Audu I, Oloso A. and Umar B.(2004).Development of a concentric cylinder Locust bean dehuller. Agricultural Engineering International: The CIGR Journal of scientific research and development manuscript PM 04003.1(11)
Barrow GI and Feltham RKA. (1993) Cowan and steel’s manual for the identification of medical bacteria. Cambridge, Cambridge University press.
Chotwanawirach TA. (1980). Microbiological study on Thai traditional fermented food products, M.Sc. Thesis, Kasetsart University, Thailand.
Claus D and Berkerly RCW (1986). The genus Bacillus in Bergey’s manual of systematic Bacteriology, 2; 1105-1139
Dakwa S, Sakyi-Dawson E, Diako C, Annan NT and Amoa-Awua WK. (2005): Effect on the fermentation of soybeans into dawadawa (Soy-dawadawa). International journal food, microbiology. 104:69-82.
Edema MO and Fawole O. (2006). Evaluation and optimization of critical control points in the production of Iru. Research Journal of Microbiology, 26; 533-541
Feng XM, Erikson ARB and Schurer J (2005). Growth of lactic acid bacteria and Rhizopus oligosporus during berley tempeh fermentation. International Journal of Food Microbiology, 104:249-256.
Food and Agricultural Organisation (1979). Manuals of food Quality Control. FAO Food and Nutrition Paper, United Nations, Rome 14(4) A1-F10.
Ibrahim AD, Sani A, Aliero AA. and Shinkafi SA. (2011) Biocatalysis of H. sabdariffa during dawadawan botso production and biogenaration of volatile compounds. International journal of Biology and Chemical Science . 5(5):1922-1931.
Ihekoronye AI and Ngoddy PO. (1985). Integrated food science and technology for the tropics. Macmillan Publishers Ltd, London and Basingstroke, pp 34-87.
National Advisory Committee on Microbiology Criteria for Food (NACMF, 1997). Hazard Analysis and Critical Control Point, Principle and Guidelines.
Obeta JAN (1983) A note on the microorganisms associated with the fermentation of seeds of African oil bean (Pentaclethra macrophyla) Journal of Applied Biotechnology 54:433-435.
Odunfa SA and Komolafe OB. (1989) Nutritional characteristics of Staphylococcus species from fermenting African locust bean (Parkia biglobosa). Nahrung. 33:607–615.
Odunfa SA. (1981). Microorganisms associated with fermentation of African locust bean (Parkia filicoidea) during iru preparation. Journal of Plant Foods. 3: 245-250.
Ogunshe AAO, Omotosho MO and Ayanshina ADV. (2007). Microbial studies and biochemical characteristics of controlled fermented afiyo– a Nigerian fermented food condiment from Prosopis africana (Guill and Perr.) Taub. Pakistan Journal of Nutrition 6 (6):620-627.
Omafuvbe BO, Shonukan OO and Abiose SH (2000). Microbiological and biochemical changes in the traditional fermntation of soybean for soy- daddawa -a Nigerian food condiment. Food Microbiology. 17: 469-474.
Ouoba LI, Diawara B, Jespersen L and Jakobsen M (2007). Antimicrobial activity of Bacillus subtilis and Bacillus pumilus during the fermentation of African locust bean (Parkia biglobosa) for soumbala production. Journal of Applied Microbiology 102: 963-970 147.
Oyeyiola GP (2002). Fermentation of the seed of Prosopis Africana to produce condiment . NISEB Journal 2 (2):287-299.
Rabi M, Mukhtar MD, Kawo AH, Shamsuddeen U and Aminu B.(2013). Evaluation of the critical control points (CCP) in the production of Daddawa (African locust bean cake) Bayero Journal of pure and Applied Sciences, 6 (1):46-51.
Sadiku OA (2010): Processing Methods of Influence, the quality of fermented African Locust Bean (Iru/Ogiri/dadawa) Parkia biglobosa. Journal of Applied Sciences Research 6(11):1656-1661.
Journal Name : citation_journal : Greener Journal of Microbiology and Antimicrobials